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Structural basis for potassium transport in prokaryotes by KdpFABC.

Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
Proc Natl Acad Sci U S A 118 (2021)
Related entries: 7bh1, 7bh2, 7lc3, 7lc6

Cited: 10 times
EuropePMC logo PMID: 34272288

Abstract

KdpFABC is an oligomeric K+ transport complex in prokaryotes that maintains ionic homeostasis under stress conditions. The complex comprises a channel-like subunit (KdpA) from the superfamily of K+ transporters and a pump-like subunit (KdpB) from the superfamily of P-type ATPases. Recent structural work has defined the architecture and generated contradictory hypotheses for the transport mechanism. Here, we use substrate analogs to stabilize four key intermediates in the reaction cycle and determine the corresponding structures by cryogenic electron microscopy. We find that KdpB undergoes conformational changes consistent with other representatives from the P-type superfamily, whereas KdpA, KdpC, and KdpF remain static. We observe a series of spherical densities that we assign as K+ or water and which define a pathway for K+ transport. This pathway runs through an intramembrane tunnel in KdpA and delivers ions to sites in the membrane domain of KdpB. Our structures suggest a mechanism where ATP hydrolysis is coupled to K+ transfer between alternative sites in KdpB, ultimately reaching a low-affinity site where a water-filled pathway allows release of K+ to the cytoplasm.

Reviews - 7bgy mentioned but not cited (1)

  1. Computational molecular docking and virtual screening revealed promising SARS-CoV-2 drugs. Hosseini M, Chen W, Xiao D, Wang C. Precis Clin Med 4 1-16 (2021)

Articles - 7bgy mentioned but not cited (2)

  1. Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC. Silberberg JM, Corey RA, Hielkema L, Stock C, Stansfeld PJ, Paulino C, Hänelt I. Nat Commun 12 5098 (2021)
  2. Inhibited KdpFABC transitions into an E1 off-cycle state. Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hänelt I, Paulino C. Elife 11 e80988 (2022)


Reviews citing this publication (2)

  1. P-type ATPases: Many more enigmas left to solve. Palmgren M. J Biol Chem 299 105352 (2023)
  2. Exposing the small protein load of bacterial life. Simoens L, Fijalkowski I, Van Damme P. FEMS Microbiol Rev 47 fuad063 (2023)

Articles citing this publication (5)

  1. Structural basis of ion uptake in copper-transporting P1B-type ATPases. Salustros N, Grønberg C, Abeyrathna NS, Lyu P, Orädd F, Wang K, Andersson M, Meloni G, Gourdon P. Nat Commun 13 5121 (2022)
  2. Comparative genomics reveals intraspecific divergence of Acidithiobacillus ferrooxidans: insights from evolutionary adaptation. Liu R, Ma L, Wang H, Liu D, Lu X, Huang X, Huang S, Liu X. Microb Genom 9 (2023)
  3. Homologs of Ancestral CNNM Proteins Affect Magnesium Homeostasis and Circadian Rhythmicity in a Model Eukaryotic Cell. Gil S, Feord HK, van Ooijen G. Int J Mol Sci 24 2273 (2023)
  4. Insertion sequence contributes to the evolution and environmental adaptation of Acidithiobacillus. Huang S, Li H, Ma L, Liu R, Li Y, Wang H, Lu X, Huang X, Wu X, Liu X. BMC Genomics 24 282 (2023)
  5. The two-component system CpxAR is required for the high potassium stress survival of Actinobacillus pleuropneumoniae. Wan J, Zhang R, Jia Y, Xie T, Dai L, Yao Q, Zhang W, Xiao H, Gao X, Huang J, Bei W, Liu F. Front Microbiol 14 1259935 (2023)


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