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Crystal structure of IscU wild-type

X-ray diffraction
3.5Å resolution
PDB entry 7c8m coloured by chain, front view.
PDB entry 7c8m coloured by chain, side view.
PDB entry 7c8m coloured by chain, top view.
Source organism: Methanothrix thermoacetophila
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: NIF system FeS cluster assembly NifU N-terminal domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 12893.01 Å2
Buried surface area: 1729.48 Å2
Dissociation area: 604.48 Å2
Dissociation energy (ΔGdiss): -3.82 kcal/mol
Dissociation entropy (TΔSdiss): 11.71 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-106062

Macromolecules

NIF system FeS cluster assembly NifU N-terminal domain-containing protein
Chains: A, C
Length: 137 amino acids
Theoretical weight: 15.26 KDa
Source organism: Methanothrix thermoacetophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0B757 (Residues: 1-129; Coverage: 100%)
Gene name: Mthe_0741
Pfam: NifU-like N terminal domain
InterPro:
NIF system FeS cluster assembly NifU N-terminal domain-containing protein in PDB entry 7c8m, assembly 1, front view.
NIF system FeS cluster assembly NifU N-terminal domain-containing protein in PDB entry 7c8m, assembly 1, side view.
NIF system FeS cluster assembly NifU N-terminal domain-containing protein in PDB entry 7c8m, assembly 1, top view.
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