› 7d0p

X-ray diffraction
1.8Å resolution

Crystal structure of human HBO1-BRPF2 in complex with propionyl-coenzyme A

Released:
DOI: 10.2210/pdb7d0p/pdb
PDB entry 7d0p coloured by chain, front view.
PDB entry 7d0p coloured by chain, side view.
PDB entry 7d0p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
HBO1 is a versatile histone acyltransferase critical for promoter histone acylations.
Xiao Y, Li W, Yang H, Pan L, Zhang L, Lu L, Chen J, Wei W, Ye J, Li J, Li G, Zhang Y, Tan M, Ding J, Wong J
OpenAccess logo Nucleic Acids Res 49 8037-8059 (2021)
PMID: 34259319

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131866 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone acetyltransferase KAT7 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95251 (Residues: 336-611; Coverage: 45%)
Gene names: HBO1, HBOa, KAT7, MYST2
Sequence domains:
Bromodomain-containing protein 1 Chain: B
Molecule details ›
Chain: B
Length: 50 amino acids
Theoretical weight: 5.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95696 (Residues: 31-80; Coverage: 5%)
Gene names: BRD1, BRL, BRPF2

Ligands and Environments


Cofactor: Ligand 1VU 1 x 1VU
2 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 126.679Å b: 39.306Å c: 87.744Å
α: 90° β: 123.01° γ: 90°
R-values:
R R work R free
0.173 0.171 0.226
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Oncometabolites drive tumorigenesis by enhancing protein acylation: from chromosomal remodelling to nonhistone modification.
Fu et al. (2022)
6 more

1 mention without citation

HBO1 is a versatile histone acyltransferase critical for promoter histone acylations.
Xiao et al. (2021)