› 7doh

X-ray diffraction
1.45Å resolution

Crystal Structure of GD-26 Fab in Complex with TD Peptide from Haloarcula Marismortui Bacteriorhodopsin I

Released:
DOI: 10.2210/pdb7doh/pdb
PDB entry 7doh coloured by chain, front view.
PDB entry 7doh coloured by chain, side view.
PDB entry 7doh coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of an epitope tagging system derived from Haloarcula marismortui bacteriorhodopsin I D94N and its monoclonal antibody GD-26.
Pao PJ, Hsu MF, Chiang MH, Chen CT, Lee CC, Wang AH
OpenAccess logo FEBS J 289 730-747 (2022)
PMID: 34499806

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212324 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Bacteriorhodopsin-I Chain: I
Molecule details ›
Chain: I
Length: 9 amino acids
Theoretical weight: 804 Da
Source organism: Haloarcula marismortui
Expression system: Not provided
UniProt:
  • Canonical: Q5UXY6 (Residues: 242-250; Coverage: 4%)
Gene names: bop, rrnAC3161
GD-26 Fab L-chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.31 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
GD-26 Fab H-chain Chain: H
Molecule details ›
Chain: H
Length: 227 amino acids
Theoretical weight: 24.49 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P212121
Unit cell:
a: 51.427Å b: 74.341Å c: 108.103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.13 0.178
Expression systems:
  • Not provided
  • Homo sapiens

Quick links

Citations

1 mention without citation

Structural basis of an epitope tagging system derived from Haloarcula marismortui bacteriorhodopsin I D94N and its monoclonal antibody GD-26.
Pao et al. (2022)