7dvq

Electron Microscopy
2.89Å resolution

Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)

Released:
Source organism: Homo sapiens
Primary publication:
Structure of the activated human minor spliceosome.
Science 371 (2021)
PMID: 33509932
Related structures: EMD-30875

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 49-mer (preferred)
PDBe Complex ID:
PDB-CPX-249700 (preferred)
Entry contents:
38 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (42 distinct):
Pre-mRNA-processing-splicing factor 8 Chain: A
116 kDa U5 small nuclear ribonucleoprotein component Chain: C
U5 small nuclear ribonucleoprotein 200 kDa helicase Chain: D
U5 small nuclear ribonucleoprotein 40 kDa protein Chain: E
Small nuclear ribonucleoprotein Sm D3 Chains: a, h
Small nuclear ribonucleoprotein-associated proteins B and B' Chains: b, i
Small nuclear ribonucleoprotein Sm D1 Chains: c, j
Small nuclear ribonucleoprotein Sm D2 Chains: d, k
Small nuclear ribonucleoprotein F Chains: f, m
Small nuclear ribonucleoprotein E Chains: e, l
Small nuclear ribonucleoprotein G Chains: g, n
Sodium channel modifier 1 Chain: v
Splicing factor 3B subunit 1 Chain: 1
Splicing factor 3B subunit 2 Chain: 2
Splicing factor 3B subunit 3 Chain: 3
Splicing factor 3B subunit 4 Chain: 4
Splicing factor 3B subunit 6 Chain: 5
PHD finger-like domain-containing protein 5A Chain: 6
Splicing factor 3B subunit 5 Chain: 7
Cell division cycle 5-like protein Chain: L
Crooked neck-like protein 1 Chain: J
Spliceosome-associated protein CWC15 homolog Chain: P
SNW domain-containing protein 1 Chain: R
Pleiotropic regulator 1 Chain: T
Smad nuclear-interacting protein 1 Chain: X
RNA-binding motif protein, X-linked 2 Chain: Y
BUD13 homolog Chain: Z
RING-type E3 ubiquitin-protein ligase PPIL2 Chain: 9
Spliceosome-associated protein CWC27 homolog Chain: z
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 Chain: x
G-patch domain and KOW motifs-containing protein Chain: y
E3 ubiquitin-protein ligase RNF113A Chain: M
Serine/arginine repetitive matrix protein 2 Chain: U
Pre-mRNA-splicing factor CWC22 homolog Chain: V
Serine/arginine repetitive matrix protein 1 Chain: 8
Cysteine-rich PDZ-binding protein Chain: 0
RNA-binding protein 48 Chain: I
Armadillo repeat-containing protein 7 Chain: K
U5 snRNA Chain: B
U6atac snRNA Chain: F
pre-mRNA Chain: G
U12 snRNA Chain: H

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.89Å
Relevant EMDB volumes: EMD-30875