› 7e8s

Electron Microscopy
4.36Å resolution

Intact TRAPPII (state I).

Released:
DOI: 10.2210/pdb7e8s/pdb
PDB entry 7e8s coloured by chain, front view.
PDB entry 7e8s coloured by chain, side view.
PDB entry 7e8s coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32.
Mi C, Zhang L, Huang G, Shao G, Yang F, You X, Dong MQ, Sun S, Sui SF
OpenAccess logo Sci Adv 8 eabi5603 (2022)
PMID: 35080977
Related structures: EMD-31021

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152406 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
TRAPP-associated protein TCA17 Chains: A, L
Molecule details ›
Chains: A, L
Length: 152 amino acids
Theoretical weight: 17.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32613 (Residues: 1-152; Coverage: 100%)
Gene names: SYGP-ORF36, TCA17, YEL048C
Sequence domains: Sedlin, N-terminal conserved region
Trafficking protein particle complex subunit 33 Chains: B, M
Molecule details ›
Chains: B, M
Length: 268 amino acids
Theoretical weight: 30.79 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q99394 (Residues: 1-268; Coverage: 100%)
Gene names: O3251, TRS33, YOR115C, YOR3251C
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit BET3 Chains: C, F, N, Q
Molecule details ›
Chains: C, F, N, Q
Length: 193 amino acids
Theoretical weight: 22.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P36149 (Residues: 1-193; Coverage: 100%)
Gene names: BET3, YKR068C
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit BET5 Chains: D, O
Molecule details ›
Chains: D, O
Length: 159 amino acids
Theoretical weight: 18.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03630 (Residues: 1-159; Coverage: 100%)
Gene names: BET5, YML077W
Sequence domains: Sybindin-like family
Trafficking protein particle complex subunit 23 Chains: E, P
Molecule details ›
Chains: E, P
Length: 219 amino acids
Theoretical weight: 24.89 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03784 (Residues: 1-219; Coverage: 100%)
Gene names: TRS23, YD8419.13, YDR246W
Sequence domains: Sybindin-like family
Trafficking protein particle complex subunit 31 Chains: G, R
Molecule details ›
Chains: G, R
Length: 283 amino acids
Theoretical weight: 31.74 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03337 (Residues: 1-283; Coverage: 100%)
Gene names: TRS31, YDR472W
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit 20 Chains: H, S
Molecule details ›
Chains: H, S
Length: 175 amino acids
Theoretical weight: 19.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38334 (Residues: 1-175; Coverage: 100%)
Gene names: TRS20, YBR1722, YBR254C
Sequence domains: Sedlin, N-terminal conserved region
Trafficking protein particle complex II-specific subunit 130 Chains: I, T
Molecule details ›
Chains: I, T
Length: 1102 amino acids
Theoretical weight: 128.23 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03660 (Residues: 1-1102; Coverage: 100%)
Gene names: TRS130, YM8261.12C, YMR218C
Sequence domains: Trafficking protein particle complex subunit 10, TRAPPC10
Trafficking protein particle complex II-specific subunit 120 Chains: J, U
Molecule details ›
Chains: J, U
Length: 1289 amino acids
Theoretical weight: 147.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04183 (Residues: 1-1289; Coverage: 100%)
Gene names: D9509.25, ESBP10, TRS120, YDR407C
Sequence domains: Transport protein Trs120 or TRAPPC9, TRAPP II complex subunit
Trafficking protein particle complex II-specific subunit 65 Chains: K, V
Molecule details ›
Chains: K, V
Length: 560 amino acids
Theoretical weight: 63.43 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32893 (Residues: 1-560; Coverage: 100%)
Gene names: KRE11, TRS65, YGR166W
Sequence domains: TRAPP trafficking subunit Trs65

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.36Å
Relevant EMDB volumes: EMD-31021
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

The TRAPP complexes: oligomeric exchange factors that activate the small GTPases Rab1 and Rab11.
Galindo et al. (2023)
1 more