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Intact TRAPPII (state I).

Electron Microscopy
4.36Å resolution
PDB entry 7e8s coloured by chain, front view.
PDB entry 7e8s coloured by chain, side view.
PDB entry 7e8s coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Assembly composition:
hetero 22-mer (preferred)
Entry contents: 10 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: TRAPP II complex
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Multimeric state: hetero 22-mer
Accessible surface area: 334410.19 Å2
Buried surface area: 67035.78 Å2
Dissociation area: 714.38 Å2
Dissociation energy (ΔGdiss): -12.18 kcal/mol
Dissociation entropy (TΔSdiss): 19.33 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-152406
Complex Portal ID: CPX-1939

Macromolecules

TRAPP-associated protein TCA17
Chains: A, L
Length: 152 amino acids
Theoretical weight: 17.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32613 (Residues: 1-152; Coverage: 100%)
Gene names: SYGP-ORF36, TCA17, YEL048C
Pfam: Sedlin, N-terminal conserved region
InterPro:
TRAPP-associated protein TCA17 in PDB entry 7e8s, assembly 1, front view.
TRAPP-associated protein TCA17 in PDB entry 7e8s, assembly 1, side view.
TRAPP-associated protein TCA17 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex subunit 33
Chains: B, M
Length: 268 amino acids
Theoretical weight: 30.79 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q99394 (Residues: 1-268; Coverage: 100%)
Gene names: O3251, TRS33, YOR115C, YOR3251C
Pfam: Transport protein particle (TRAPP) component
InterPro:
Trafficking protein particle complex subunit 33 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex subunit 33 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex subunit 33 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex subunit BET3
Chains: C, F, N, Q
Length: 193 amino acids
Theoretical weight: 22.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P36149 (Residues: 1-193; Coverage: 100%)
Gene names: BET3, YKR068C
Pfam: Transport protein particle (TRAPP) component
InterPro:
Trafficking protein particle complex subunit BET3 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex subunit BET3 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex subunit BET3 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex subunit BET5
Chains: D, O
Length: 159 amino acids
Theoretical weight: 18.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03630 (Residues: 1-159; Coverage: 100%)
Gene names: BET5, YML077W
Pfam: Sybindin-like family
InterPro:
Trafficking protein particle complex subunit BET5 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex subunit BET5 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex subunit BET5 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex subunit 23
Chains: E, P
Length: 219 amino acids
Theoretical weight: 24.89 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03784 (Residues: 1-219; Coverage: 100%)
Gene names: TRS23, YD8419.13, YDR246W
Pfam: Sybindin-like family
InterPro:
Trafficking protein particle complex subunit 23 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex subunit 23 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex subunit 23 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex subunit 31
Chains: G, R
Length: 283 amino acids
Theoretical weight: 31.74 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03337 (Residues: 1-283; Coverage: 100%)
Gene names: TRS31, YDR472W
Pfam: Transport protein particle (TRAPP) component
InterPro:
Trafficking protein particle complex subunit 31 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex subunit 31 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex subunit 31 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex subunit 20
Chains: H, S
Length: 175 amino acids
Theoretical weight: 19.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38334 (Residues: 1-175; Coverage: 100%)
Gene names: TRS20, YBR1722, YBR254C
Pfam: Sedlin, N-terminal conserved region
InterPro:
Trafficking protein particle complex subunit 20 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex subunit 20 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex subunit 20 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex II-specific subunit 130
Chains: I, T
Length: 1102 amino acids
Theoretical weight: 128.23 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03660 (Residues: 1-1102; Coverage: 100%)
Gene names: TRS130, YM8261.12C, YMR218C
Pfam: Trafficking protein particle complex subunit 10, TRAPPC10
InterPro:
Trafficking protein particle complex II-specific subunit 130 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex II-specific subunit 130 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex II-specific subunit 130 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex II-specific subunit 120
Chains: J, U
Length: 1289 amino acids
Theoretical weight: 147.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04183 (Residues: 1-1289; Coverage: 100%)
Gene names: D9509.25, ESBP10, TRS120, YDR407C
Pfam: Transport protein Trs120 or TRAPPC9, TRAPP II complex subunit
InterPro: TRAPP II complex, Trs120
Trafficking protein particle complex II-specific subunit 120 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex II-specific subunit 120 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex II-specific subunit 120 in PDB entry 7e8s, assembly 1, top view.
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Trafficking protein particle complex II-specific subunit 65
Chains: K, V
Length: 560 amino acids
Theoretical weight: 63.43 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32893 (Residues: 1-560; Coverage: 100%)
Gene names: KRE11, TRS65, YGR166W
Pfam: TRAPP trafficking subunit Trs65
InterPro: Trafficking protein particle complex II-specific subunit 65
Trafficking protein particle complex II-specific subunit 65 in PDB entry 7e8s, assembly 1, front view.
Trafficking protein particle complex II-specific subunit 65 in PDB entry 7e8s, assembly 1, side view.
Trafficking protein particle complex II-specific subunit 65 in PDB entry 7e8s, assembly 1, top view.
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