› 7ef0

X-ray diffraction
1.5Å resolution

Crystal structure of maize SHH2 SAWADEE domain in complex with an H3K9M peptide

Released:
DOI: 10.2210/pdb7ef0/pdb
PDB entry 7ef0 coloured by chain, front view.
PDB entry 7ef0 coloured by chain, side view.
PDB entry 7ef0 coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2.
Wang Y, Zhou X, Luo J, Lv S, Liu R, Du X, Jia B, Yuan F, Zhang H, Du J
J Integr Plant Biol 63 1091-1096 (2021)
PMID: 33913587

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-110665 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Homeobox domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 amino acids
Theoretical weight: 18.1 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7ZYP9 (Residues: 125-281; Coverage: 41%)
Gene names: 100279552, HB131, ZEAMMB73_Zm00001d005584
Sequence domains: SAWADEE domain
Histone H3.2 Chain: P
Molecule details ›
Chain: P
Length: 19 amino acids
Theoretical weight: 2.08 KDa
Source organism: Zea mays
Expression system: Not provided
UniProt:
  • Canonical: P69246 (Residues: 2-20; Coverage: 14%)
Gene names: H3C2, H3C3, H3C4

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 46.405Å b: 50.032Å c: 133.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.208
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Reprogramming of Histone H3 Lysine Methylation During Plant Sexual Reproduction.
Fang et al. (2021)
3 other articles