› 7efo

Electron Microscopy
3.85Å resolution

LptB2FG-LPS from Klebsiella pneumoniae in nanodiscs

Released:
DOI: 10.2210/pdb7efo/pdb
PDB entry 7efo coloured by chain, front view.
PDB entry 7efo coloured by chain, side view.
PDB entry 7efo coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Primary publication:
Cryo-EM structures of LptB2FG and LptB2FGC from Klebsiella pneumoniae in complex with lipopolysaccharide.
Luo Q, Shi H, Xu X
Biochem Biophys Res Commun 571 20-25 (2021)
PMID: 34303191
Related structures: EMD-31088

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-103885 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lipopolysaccharide export system ATP-binding protein LptB Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 26.88 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2X3II39 (Residues: 1-241; Coverage: 100%)
Gene names: E9161_06020, GLO21_00280, GLO21_023485, IUJ34_15505, NCTC9504_00934, lptB, lptB_3
Sequence domains:
Lipopolysaccharide export system permease protein LptF Chain: F
Molecule details ›
Chain: F
Length: 365 amino acids
Theoretical weight: 40.43 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Y0Q3P9 (Residues: 1-365; Coverage: 100%)
Gene names: E9161_18815, GLO21_002610, GLO21_13830, NCTC9504_05841, lptF
Sequence domains: Lipopolysaccharide export system permease LptF/LptG
LPS export ABC transporter permease LptG Chain: G
Molecule details ›
Chain: G
Length: 360 amino acids
Theoretical weight: 39.66 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Y0Q3C0 (Residues: 1-360; Coverage: 100%)
Gene names: E9161_18820, GLO21_002615, GLO21_13825, NCTC9504_05840, lptG
Sequence domains: Lipopolysaccharide export system permease LptF/LptG

Ligands and Environments

1 bound ligand:
Ligand JSG 1 x JSG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.85Å
Relevant EMDB volumes: EMD-31088
Expression system: Escherichia coli

Quick links

Citations

1 review citation

ABC Transporters in Bacterial Nanomachineries.
Bilsing et al. (2023)
5 other articles