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LptB2FG-LPS from Klebsiella pneumoniae in nanodiscs

Electron Microscopy
3.85Å resolution
PDB entry 7efo coloured by chain, front view.
PDB entry 7efo coloured by chain, side view.
PDB entry 7efo coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Assembly composition:
hetero tetramer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 40573.78 Å2
Buried surface area: 7908.19 Å2
Dissociation area: 2,573.95 Å2
Dissociation energy (ΔGdiss): 6.34 kcal/mol
Dissociation entropy (TΔSdiss): 26.97 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-103885

Macromolecules

Lipopolysaccharide export system ATP-binding protein LptB
Chains: A, B
Length: 241 amino acids
Theoretical weight: 26.88 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2X3II39 (Residues: 1-241; Coverage: 100%)
Gene names: E9161_06020, GLO21_00280, GLO21_023485, IUJ34_15505, NCTC9504_00934, lptB, lptB_3
Pfam:
InterPro:
Lipopolysaccharide export system ATP-binding protein LptB in PDB entry 7efo, assembly 1, front view.
Lipopolysaccharide export system ATP-binding protein LptB in PDB entry 7efo, assembly 1, side view.
Lipopolysaccharide export system ATP-binding protein LptB in PDB entry 7efo, assembly 1, top view.
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Lipopolysaccharide export system permease protein LptF
Chain: F
Length: 365 amino acids
Theoretical weight: 40.43 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Y0Q3P9 (Residues: 1-365; Coverage: 100%)
Gene names: E9161_18815, GLO21_002610, GLO21_13830, NCTC9504_05841, lptF
Pfam: Lipopolysaccharide export system permease LptF/LptG
InterPro:
Lipopolysaccharide export system permease protein LptF in PDB entry 7efo, assembly 1, front view.
Lipopolysaccharide export system permease protein LptF in PDB entry 7efo, assembly 1, side view.
Lipopolysaccharide export system permease protein LptF in PDB entry 7efo, assembly 1, top view.
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LPS export ABC transporter permease LptG
Chain: G
Length: 360 amino acids
Theoretical weight: 39.66 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Y0Q3C0 (Residues: 1-360; Coverage: 100%)
Gene names: E9161_18820, GLO21_002615, GLO21_13825, NCTC9504_05840, lptG
Pfam: Lipopolysaccharide export system permease LptF/LptG
InterPro:
Permease in PDB entry 7efo, assembly 1, front view.
Permease in PDB entry 7efo, assembly 1, side view.
Permease in PDB entry 7efo, assembly 1, top view.
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