› 7f55

Electron Microscopy
3.1Å resolution

Cryo-EM structure of bremelanotide-MC4R-Gs_Nb35 complex

Released:
DOI: 10.2210/pdb7f55/pdb
PDB entry 7f55 coloured by chain, front view.
PDB entry 7f55 coloured by chain, side view.
PDB entry 7f55 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into ligand recognition and activation of the melanocortin-4 receptor.
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res 31 1163-1175 (2021)
PMID: 34433901
Related structures: EMD-31458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211060 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda ascovirus 1c
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 384 amino acids
Theoretical weight: 41.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Melanocortin receptor 4 Chain: R
Molecule details ›
Chain: R
Length: 507 amino acids
Theoretical weight: 56.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32245 (Residues: 1-332; Coverage: 100%)
Gene name: MC4R
Sequence domains: 7 transmembrane receptor (rhodopsin family)
bremelanotide Chain: L
Molecule details ›
Chain: L
Length: 7 amino acids
Theoretical weight: 1 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
2 modified residues:
Ligand NLE 1 x NLE
Ligand DPN 1 x DPN

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-31458
Expression systems:
  • Spodoptera frugiperda ascovirus 1c
  • Spodoptera frugiperda
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Lipocalin 2 receptors: facts, fictions, and myths.
Schröder et al. (2023)
6 other articles

3 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
2 more