› 7jr7

Electron Microscopy
3.3Å resolution

Cryo-EM structure of ABCG5/G8 in complex with Fab 2E10 and 11F4

Released:
DOI: 10.2210/pdb7jr7/pdb
PDB entry 7jr7 coloured by chain, front view.
PDB entry 7jr7 coloured by chain, side view.
PDB entry 7jr7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of ABCG5/G8 in complex with modulating antibodies
Zhang H, Huang CS, Yu X, Lee J, Vaish A, Chen Q, Zhou M, Wang Z, Min X
Commun Biol 4 526- (2021)
Related structures: EMD-22443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-212723 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
ATP-binding cassette sub-family G member 5 Chain: A
Molecule details ›
Chain: A
Length: 651 amino acids
Theoretical weight: 72.58 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9H222 (Residues: 1-651; Coverage: 100%)
Gene name: ABCG5
Sequence domains:
ATP-binding cassette sub-family G member 8 Chain: B
Molecule details ›
Chain: B
Length: 673 amino acids
Theoretical weight: 75.75 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9H221 (Residues: 1-673; Coverage: 100%)
Gene name: ABCG8
Sequence domains:
Fab 11F4 heavy chain Chain: C
Molecule details ›
Chain: C
Length: 220 amino acids
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab 11F4 light chain Chain: D
Molecule details ›
Chain: D
Length: 213 amino acids
Theoretical weight: 23.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab 2E10 heavy chain Chain: E
Molecule details ›
Chain: E
Length: 223 amino acids
Theoretical weight: 24.13 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab 2E10 light chain Chain: F
Molecule details ›
Chain: F
Length: 212 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-22443
Expression systems:
  • Komagataella pastoris
  • Homo sapiens

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