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Phospholipase D engineered mutant bound to phosphatidic acid (8 hour soak)

X-ray diffraction
2.21Å resolution
PDB entry 7jru coloured by chain, front view.
PDB entry 7jru coloured by chain, side view.
PDB entry 7jru coloured by chain, top view.
Source organism: Streptomyces antibioticus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Phospholipase D
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Multimeric state: monomeric
Accessible surface area: 17994.42 Å2
Buried surface area: 623.62 Å2
Dissociation area: 173.1 Å2
Dissociation energy (ΔGdiss): -7.54 kcal/mol
Dissociation entropy (TΔSdiss): 2.81 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176073

Macromolecules

Phospholipase D
Chain: A
Length: 509 amino acids
Theoretical weight: 54 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53728 (Residues: 48-556; Coverage: 100%)
Pfam: PLD-like domain
InterPro:
Phospholipase D in PDB entry 7jru, assembly 1, front view.
Phospholipase D in PDB entry 7jru, assembly 1, side view.
Phospholipase D in PDB entry 7jru, assembly 1, top view.
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