› 7jz2

Electron Microscopy
2.5Å resolution

Succinate: quinone oxidoreductase SQR from E.coli K12

Released:
DOI: 10.2210/pdb7jz2/pdb
PDB entry 7jz2 coloured by chain, front view.
PDB entry 7jz2 coloured by chain, side view.
PDB entry 7jz2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.
Su CC, Lyu M, Morgan CE, Bolla JR, Robinson CV, Yu EW
Nat Methods 18 69-75 (2021)
PMID: 33408407
Related structures: EMD-22528

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-139347 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Succinate dehydrogenase flavoprotein subunit Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 588 amino acids
Theoretical weight: 64.5 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0AC41 (Residues: 1-588; Coverage: 100%)
Gene names: JW0713, b0723, sdhA
Sequence domains:
Succinate dehydrogenase iron-sulfur subunit Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 238 amino acids
Theoretical weight: 26.8 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P07014 (Residues: 1-238; Coverage: 100%)
Gene names: JW0714, b0724, sdhB
Sequence domains:
Succinate dehydrogenase cytochrome b556 subunit Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 129 amino acids
Theoretical weight: 14.31 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P69054 (Residues: 1-129; Coverage: 100%)
Gene names: JW0711, b0721, cybA, sdhC
Sequence domains: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Succinate dehydrogenase hydrophobic membrane anchor subunit Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 115 amino acids
Theoretical weight: 12.87 KDa
Source organism: Escherichia coli K-12

Ligands and Environments


Cofactor: Ligand UQ2 3 x UQ2

Cofactor: Ligand HEM 3 x HEM

Cofactor: Ligand FAD 3 x FAD
5 bound ligands:
Ligand NA 3 x NA
Ligand FES 3 x FES
Ligand SF4 3 x SF4
Ligand F3S 3 x F3S
Ligand 3PE 3 x 3PE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-22528

Quick links

Citations

11 review citations

Approaches to Heterogeneity in Native Mass Spectrometry.
Rolland et al. (2022)
10 more

1 mention without citation

A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.
Su et al. (2021)