› 7kbp

X-ray diffraction
2.3Å resolution

Herceptin Diabody with R83T, E85C Mutations

Released:
DOI: 10.2210/pdb7kbp/pdb
PDB entry 7kbp coloured by chain, front view.
PDB entry 7kbp coloured by chain, side view.
PDB entry 7kbp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Co-crystallization with diabodies: A case study for the introduction of synthetic symmetry.
Chesterman C, Arnold E
Structure 29 598-605.e3 (2021)
PMID: 33636101

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-chain Fv Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 241 amino acids
Theoretical weight: 26.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand GOL 17 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 71.153Å b: 130.924Å c: 139.999Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.232
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Modeling and Structure Determination of Homo-Oligomeric Proteins: An Overview of Challenges and Current Approaches.
Gaber et al. (2021)
1 more

5 mentions without citation

A high quality assembly of the Nile Tilapia (Oreochromis niloticus) genome reveals the structure of two sex determination regions.
Conte et al. (2017)
4 more