› 7kzm

Electron Microscopy
7.5Å resolution

Outer dynein arm bound to doublet microtubules from C. reinhardtii

Released:
DOI: 10.2210/pdb7kzm/pdb
PDB entry 7kzm coloured by chain, front view.
PDB entry 7kzm coloured by chain, side view.
PDB entry 7kzm coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structure of a microtubule-bound axonemal dynein.
Walton T, Wu H, Brown A
OpenAccess logo Nat Commun 12 477 (2021)
PMID: 33473120
Related structures: EMD-23082

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 37-mer (preferred)
PDBe Complex ID:
PDB-CPX-104306 (preferred)
Entry contents:
20 distinct polypeptide molecules
Macromolecules (20 distinct):
Tubulin beta-1/beta-2 chain Chains: A1, A3, A5, A7, B1, B3, B5, B7
Molecule details ›
Chains: A1, A3, A5, A7, B1, B3, B5, B7
Length: 443 amino acids
Theoretical weight: 49.67 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P04690 (Residues: 1-443; Coverage: 100%)
Gene names: TUBB1, TUBB2
Sequence domains:
Tubulin alpha-1 chain Chains: A2, A4, A6, B2, B4, B6
Molecule details ›
Chains: A2, A4, A6, B2, B4, B6
Length: 451 amino acids
Theoretical weight: 49.64 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P09204 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1
Sequence domains:
PLD phosphodiesterase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 4503 amino acids
Theoretical weight: 504.95 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3DV97 (Residues: 1-4503; Coverage: 100%)
Gene name: CHLRE_03g145127v5
Sequence domains:
Flagellar outer dynein arm heavy chain beta Chain: B
Molecule details ›
Chain: B
Length: 4568 amino acids
Theoretical weight: 520.51 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8J1M5 (Residues: 1-4568; Coverage: 100%)
Gene name: CHLRE_09g403800v5
Sequence domains:
Dynein gamma chain, flagellar outer arm Chain: C
Molecule details ›
Chain: C
Length: 4485 amino acids
Theoretical weight: 513.49 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q39575 (Residues: 1-4485; Coverage: 100%)
Gene names: ODA-2, ODA2
Sequence domains:
Dynein, 78 kDa intermediate chain, flagellar outer arm Chain: D
Molecule details ›
Chain: D
Length: 683 amino acids
Theoretical weight: 76.63 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q39578 (Residues: 1-683; Coverage: 100%)
Gene names: ODA-9, ODA9
Sequence domains: WD domain, G-beta repeat
Dynein, 70 kDa intermediate chain, flagellar outer arm Chain: E
Molecule details ›
Chain: E
Length: 567 amino acids
Theoretical weight: 63.59 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P27766 (Residues: 1-567; Coverage: 100%)
Gene names: IC70, ODA-6, ODA6
Mr19,000 outer arm dynein light chain Chain: F
Molecule details ›
Chain: F
Length: 136 amino acids
Theoretical weight: 15.9 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: O04355 (Residues: 1-136; Coverage: 100%)
Gene name: CHLRE_12g527750v5
Sequence domains: Tctex-1 family
Dynein 18 kDa light chain, flagellar outer arm Chain: G
Molecule details ›
Chain: G
Length: 159 amino acids
Theoretical weight: 17.81 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q39584 (Residues: 1-159; Coverage: 100%)
Sequence domains: EF-hand domain pair
Dynein 11 kDa light chain, flagellar outer arm Chain: H
Molecule details ›
Chain: H
Length: 120 amino acids
Theoretical weight: 13.88 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q39579 (Residues: 1-120; Coverage: 100%)
Sequence domains: Dynein light chain type 1
Dynein light chain roadblock Chain: I
Molecule details ›
Chain: I
Length: 105 amino acids
Theoretical weight: 11.95 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q9SWQ6 (Residues: 1-105; Coverage: 100%)
Gene names: CHLRE_08g376550v5, CHLRE_33g759047v5, LC7
Sequence domains: Roadblock/LC7 domain
Dynein light chain roadblock Chain: J
Molecule details ›
Chain: J
Length: 100 amino acids
Theoretical weight: 11.13 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IY95 (Residues: 1-100; Coverage: 100%)
Gene name: CHLRE_12g546400v5
Sequence domains: Roadblock/LC7 domain
Dynein 8 kDa light chain, flagellar outer arm Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 91 amino acids
Theoretical weight: 10.34 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q39580 (Residues: 1-91; Coverage: 100%)
Sequence domains: Dynein light chain type 1
Dynein light chain 9 Chain: O
Molecule details ›
Chain: O
Length: 117 amino acids
Theoretical weight: 12.99 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q2VIY5 (Residues: 1-117; Coverage: 100%)
Gene name: CHLRE_10g428850v5
Sequence domains: Tctex-1 family
Dynein light chain Chain: P
Molecule details ›
Chain: P
Length: 103 amino acids
Theoretical weight: 12.1 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8J5C4 (Residues: 1-103; Coverage: 100%)
Gene names: CHLRE_12g527800v5, LC10
Sequence domains: Dynein light chain type 1
Outer dynein arm-docking complex subunit 1 Chains: X, X1
Molecule details ›
Chains: X, X1
Length: 749 amino acids
Theoretical weight: 83.52 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IPZ5 (Residues: 1-749; Coverage: 100%)
Gene name: CHLRE_17g703850v5
DC1 Chain: X0
Molecule details ›
Chain: X0
Length: 162 amino acids
Theoretical weight: 13.81 KDa
Source organism: Chlamydomonas reinhardtii
Outer dynein arm protein 1 Chains: Y, Y1
Molecule details ›
Chains: Y, Y1
Length: 552 amino acids
Theoretical weight: 62.29 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JF70 (Residues: 1-552; Coverage: 100%)
Gene names: CHLREDRAFT_132719, DCC2, ODA1
Sequence domains: ODAD1 central coiled coil region
DC2 Chain: Y0
Molecule details ›
Chain: Y0
Length: 168 amino acids
Theoretical weight: 14.32 KDa
Source organism: Chlamydomonas reinhardtii
EF-hand domain-containing protein Chain: Z
Molecule details ›
Chain: Z
Length: 184 amino acids
Theoretical weight: 21.37 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q7Y0H2 (Residues: 1-184; Coverage: 100%)
Gene names: CHLRE_14g617550v5, ODA14

Ligands and Environments

3 bound ligands:
Ligand GTP 7 x GTP
Ligand MG 7 x MG
Ligand GDP 8 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.5Å
Relevant EMDB volumes: EMD-23082

Quick links

Citations

4 review citations

Composition and function of ciliary inner-dynein-arm subunits studied in Chlamydomonas reinhardtii.
Yamamoto et al. (2021)
3 more

5 mentions without citation

De novo identification of mammalian ciliary motility proteins using cryo-EM.
Gui et al. (2021)
4 more