› 7l4e

X-ray diffraction
2Å resolution

Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C16:1-crypto Acyl Carrier Protein, AcpP

Released:
DOI: 10.2210/pdb7l4e/pdb
PDB entry 7l4e coloured by chain, front view.
PDB entry 7l4e coloured by chain, side view.
PDB entry 7l4e coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure and Mechanistic Analyses of the Gating Mechanism of Elongating Ketosynthases
Mindrebo JT, Chen A, Kim WE, Re RN, Davis TD, Noel JP, Burkart MD
ACS Catalysis 11 6787-6799 (2021)

Function and Biology Details

Reaction catalysed: 
A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141282 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-oxoacyl-[acyl-carrier-protein] synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 43.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AAI5 (Residues: 1-413; Coverage: 100%)
Gene names: JW1081, b1095, fabF, fabJ
Sequence domains:
Acyl carrier protein Chain: B
Molecule details ›
Chain: B
Length: 78 amino acids
Theoretical weight: 8.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6A8 (Residues: 1-78; Coverage: 100%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand MU4 1 x MU4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P43212
Unit cell:
a: 86.95Å b: 86.95Å c: 114.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.167 0.207
Expression system: Escherichia coli BL21(DE3)

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