› 7l7i

Electron Microscopy
3.3Å resolution

Cryo-EM structure of Hsp90:FKBP51:p23 closed-state complex

Released:
DOI: 10.2210/pdb7l7i/pdb
PDB entry 7l7i coloured by chain, front view.
PDB entry 7l7i coloured by chain, side view.
PDB entry 7l7i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of an Hsp90-immunophilin complex reveals cochaperone recognition of the client maturation state.
Lee K, Thwin AC, Nadel CM, Tse E, Gates SN, Gestwicki JE, Southworth DR
Mol Cell 81 3496-3508.e5 (2021)
PMID: 34380015
Related structures: EMD-23213

Function and Biology Details

Reactions catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
ATP + H(2)O = ADP + phosphate
(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-139825 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Peptidyl-prolyl cis-trans isomerase FKBP5 Chain: C
Molecule details ›
Chain: C
Length: 457 amino acids
Theoretical weight: 51.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13451 (Residues: 1-457; Coverage: 100%)
Gene names: AIG6, FKBP5, FKBP51
Sequence domains:
Heat shock protein HSP 90-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 732 amino acids
Theoretical weight: 84.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07900 (Residues: 1-732; Coverage: 100%)
Gene names: HSP90A, HSP90AA1, HSPC1, HSPCA
Sequence domains:
Prostaglandin E synthase 3 Chain: E
Molecule details ›
Chain: E
Length: 160 amino acids
Theoretical weight: 18.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15185 (Residues: 1-160; Coverage: 100%)
Gene names: P23, PTGES3, TEBP
Sequence domains: CS domain

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-23213
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural and functional complexity of HSP90 in cellular homeostasis and disease.
Chiosis et al. (2023)
4 more

8 mentions without citation

Advances towards Understanding the Mechanism of Action of the Hsp90 Complex.
Prodromou et al. (2022)
7 more