7lcg

Electron Microscopy
2.42Å resolution

The mature Usutu SAAR-1776, Model A

Released:
Source organism: Usutu virus
Primary publication:
Structure of Usutu virus SAAR-1776 displays fusion loop asymmetry.
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34417300
Related structures: EMD-23272

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178478 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Envelope protein E Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 500 amino acids
Theoretical weight: 53.62 KDa
Source organism: Usutu virus
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: Q5WPU5 (Residues: 294-793; Coverage: 15%)
Sequence domains:
Small envelope protein M Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 75 amino acids
Theoretical weight: 8.33 KDa
Source organism: Usutu virus
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: Q5WPU5 (Residues: 219-293; Coverage: 2%)
Sequence domains: Flavivirus envelope glycoprotein M

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.42Å
Relevant EMDB volumes: EMD-23272
Expression system: Chlorocebus aethiops