› 7lg5

Electron Microscopy
2.63Å resolution

Synechocystis sp. UTEX2470 Cyanophycin synthetase 1 with ATP

Released:
DOI: 10.2210/pdb7lg5/pdb
PDB entry 7lg5 coloured by chain, front view.
PDB entry 7lg5 coloured by chain, side view.
PDB entry 7lg5 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6714
Primary publication:
Structures and function of the amino acid polymerase cyanophycin synthetase.
Sharon I, Haque AS, Grogg M, Lahiri I, Seebach D, Leschziner AE, Hilvert D, Schmeing TM
Nat Chem Biol 17 1101-1110 (2021)
PMID: 34385683
Related structures: EMD-23311

Function and Biology Details

Reactions catalysed: 
ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp
ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100396 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanophycin synthetase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 879 amino acids
Theoretical weight: 95.76 KDa
Source organism: Synechocystis sp. PCC 6714
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A068N621 (Residues: 1-873; Coverage: 100%)
Gene names: D082_30240, cphA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 8 x ATP
Ligand MG 12 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.63Å
Relevant EMDB volumes: EMD-23311
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Cyanophycin Modifications-Widening the Application Potential.
Kwiatos et al. (2021)
9 other articles

1 mention without citation

A cryptic third active site in cyanophycin synthetase creates primers for polymerization.
Sharon et al. (2022)