› 7lle

X-ray diffraction
1.7Å resolution

Crystal structure of GenB4 in complex with PLP

Released:
DOI: 10.2210/pdb7lle/pdb
PDB entry 7lle coloured by chain, front view.
PDB entry 7lle coloured by chain, side view.
PDB entry 7lle coloured by chain, top view.
Source organism: Micromonospora echinospora
Primary publication:
Crystal structure of GenB4 in complex with external aldimine of PLP-sisomicin
Bury PS, Huang F, Leadlay PF, Dias MVB
ACS Catalysis (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative gentamicin aminotransferase IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 49.09 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6QVT7 (Residues: 1-445; Coverage: 100%)
Gene names: genB4, gntH
Sequence domains: Aminotransferase class-III

Ligands and Environments

1 bound ligand:
Ligand PLP 2 x PLP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 71Å b: 74.21Å c: 181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.154 0.184
Expression system: Escherichia coli

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