7lum › Citations

Structural and functional dissection of reovirus capsid folding and assembly by the prefoldin-TRiC/CCT chaperone network.

Knowlton JJ, Gestaut D, Ma B, Taylor G, Seven AB, Leitner A, Wilson GJ, Shanker S, Yates NA, Prasad BVV, Aebersold R, Chiu W, Frydman J, Dermody TS
Proc Natl Acad Sci U S A 118 (2021)
Cited: 18 times
EuropePMC logo PMID: 33836586

Abstract

Intracellular protein homeostasis is maintained by a network of chaperones that function to fold proteins into their native conformation. The eukaryotic TRiC chaperonin (TCP1-ring complex, also called CCT for cytosolic chaperonin containing TCP1) facilitates folding of a subset of proteins with folding constraints such as complex topologies. To better understand the mechanism of TRiC folding, we investigated the biogenesis of an obligate TRiC substrate, the reovirus σ3 capsid protein. We discovered that the σ3 protein interacts with a network of chaperones, including TRiC and prefoldin. Using a combination of cryoelectron microscopy, cross-linking mass spectrometry, and biochemical approaches, we establish functions for TRiC and prefoldin in folding σ3 and promoting its assembly into higher-order oligomers. These studies illuminate the molecular dynamics of σ3 folding and establish a biological function for TRiC in virus assembly. In addition, our findings provide structural and functional insight into the mechanism by which TRiC and prefoldin participate in the assembly of protein complexes.

Reviews - 7lum mentioned but not cited (1)

  1. Structural and Kinetic Views of Molecular Chaperones in Multidomain Protein Folding. Kawagoe S, Ishimori K, Saio T. Int J Mol Sci 23 2485 (2022)

Articles - 7lum mentioned but not cited (6)

  1. Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT. Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J. Cell 185 4770-4787.e20 (2022)
  2. Chaperone requirements for de novo folding of Saccharomyces cerevisiae septins. Hassell D, Denney A, Singer E, Benson A, Roth A, Ceglowski J, Steingesser M, McMurray M. Mol Biol Cell 33 ar111 (2022)
  3. Mechanism of orphan subunit recognition during assembly quality control. Yagita Y, Zavodszky E, Peak-Chew SY, Hegde RS. Cell 186 3443-3459.e24 (2023)
  4. Identification of sitagliptin binding proteins by affinity purification mass spectrometry. Wang XN, Sim BR, Chen H, Zheng YX, Xue JB, Wang L, Kong WS, Zhou K, Guo SJ, Hou JL, Zhang J, Jiang HW, Tao SC. Acta Biochim Biophys Sin (Shanghai) 54 1453-1463 (2022)
  5. A structural vista of phosducin-like PhLP2A-chaperonin TRiC cooperation during the ATP-driven folding cycle. Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH. Nat Commun 15 1007 (2024)
  6. Reduced ADP off-rate by the yeast CCT2 double mutation T394P/R510H which causes Leber congenital amaurosis in humans. Roy M, Fleisher RC, Alexandrov AI, Horovitz A. Commun Biol 6 888 (2023)


Reviews citing this publication (4)

  1. Prefoldin Function in Cellular Protein Homeostasis and Human Diseases. Tahmaz I, Shahmoradi Ghahe S, Topf U. Front Cell Dev Biol 9 816214 (2021)
  2. Mechanistic insights into protein folding by the eukaryotic chaperonin complex CCT. Smith TM, Willardson BM. Biochem Soc Trans 50 1403-1414 (2022)
  3. The chaperone system in cancer therapies: Hsp90. Basset CA, Conway de Macario E, Leone LG, Macario AJL, Leone A. J Mol Histol 54 105-118 (2023)
  4. Protein Quality Control Systems and ER Stress as Key Players in SARS-CoV-2-Induced Neurodegeneration. Gavilán E, Medina-Guzman R, Bahatyrevich-Kharitonik B, Ruano D. Cells 13 123 (2024)

Articles citing this publication (7)

  1. Snapshots of actin and tubulin folding inside the TRiC chaperonin. Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW. Nat Struct Mol Biol 29 420-429 (2022)
  2. Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly. Kniert J, Dos Santos T, Eaton HE, Jung Cho W, Plummer G, Shmulevitz M. PLoS Pathog 18 e1010641 (2022)
  3. A CRISPR-Cas9 screen reveals a role for WD repeat-containing protein 81 (WDR81) in the entry of late penetrating viruses. Snyder AJ, Abad AT, Danthi P. PLoS Pathog 18 e1010398 (2022)
  4. Pathway and mechanism of tubulin folding mediated by TRiC/CCT along its ATPase cycle revealed using cryo-EM. Liu C, Jin M, Wang S, Han W, Zhao Q, Wang Y, Xu C, Diao L, Yin Y, Peng C, Peng C, Bao L, Wang Y, Cong Y. Commun Biol 6 531 (2023)
  5. Structural basis of plp2-mediated cytoskeletal protein folding by TRiC/CCT. Han W, Jin M, Liu C, Zhao Q, Wang S, Wang Y, Yin Y, Peng C, Wang Y, Cong Y. Sci Adv 9 eade1207 (2023)
  6. A genome-wide screen identifies SCAI as a modulator of the UV-induced replicative stress response. Lemay JF, St-Hilaire E, Ronato DA, Gao Y, Bélanger F, Gezzar-Dandashi S, Kimenyi Ishimwe AB, Sawchyn C, Lévesque D, McQuaid M, Boisvert FM, Mallette FA, Masson JY, Drobetsky EA, Wurtele H. PLoS Biol 20 e3001543 (2022)
  7. other 14th International dsRNA Virus Symposium, Banff, Alberta, Canada, 10-14 October 2022. Desselberger U. Virus Res 324 199032 (2023)


Quick links