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SARS-CoV-2 Spike:5A6 Fab complex I focused refinement

Electron Microscopy
2.66Å resolution
PDB entry 7m71 coloured by chain, front view.
PDB entry 7m71 coloured by chain, side view.
PDB entry 7m71 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Spike protein S2' and IG-heavy chain and IG-light kappa chain
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Multimeric state: hetero tetramer
Accessible surface area: 40589.09 Å2
Buried surface area: 7463.68 Å2
Dissociation area: 357.54 Å2
Dissociation energy (ΔGdiss): -9.02 kcal/mol
Dissociation entropy (TΔSdiss): 13.42 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-210102

Macromolecules

Spike glycoprotein
Chains: A, B
Length: 1208 amino acids
Theoretical weight: 133.75 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Pfam:
InterPro:
Spike glycoprotein in PDB entry 7m71, assembly 1, front view.
Spike glycoprotein in PDB entry 7m71, assembly 1, side view.
Spike glycoprotein in PDB entry 7m71, assembly 1, top view.
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Antibody 5A6 Fab heavy chain
Chain: H
Length: 449 amino acids
Theoretical weight: 49.17 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
InterPro:
Antibody 5A6 Fab heavy chain in PDB entry 7m71, assembly 1, front view.
Antibody 5A6 Fab heavy chain in PDB entry 7m71, assembly 1, side view.
Antibody 5A6 Fab heavy chain in PDB entry 7m71, assembly 1, top view.
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Antibody 5A6 Fab light chain
Chain: L
Length: 214 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
InterPro:
Antibody 5A6 Fab light chain in PDB entry 7m71, assembly 1, front view.
Antibody 5A6 Fab light chain in PDB entry 7m71, assembly 1, side view.
Antibody 5A6 Fab light chain in PDB entry 7m71, assembly 1, top view.
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