› 7mhy

Electron Microscopy
2.7Å resolution

Human Hedgehog acyltransferase (HHAT) in complex with palmitoyl-CoA and two Fab antibody fragments

Released:
DOI: 10.2210/pdb7mhy/pdb
PDB entry 7mhy coloured by chain, front view.
PDB entry 7mhy coloured by chain, side view.
PDB entry 7mhy coloured by chain, top view.
Source organisms:
Primary publication:
Substrate and product complexes reveal mechanisms of Hedgehog acylation by HHAT.
Jiang Y, Benz TL, Long SB
Science 372 1215-1219 (2021)
PMID: 34112694
Related structures: EMD-23836

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212325 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Protein-cysteine N-palmitoyltransferase HHAT Chain: A
Molecule details ›
Chain: A
Length: 493 amino acids
Theoretical weight: 57.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5VTY9 (Residues: 1-493; Coverage: 100%)
Gene names: HHAT, MART2, SKI1
Sequence domains: MBOAT, membrane-bound O-acyltransferase family
1C06 Fab heavy chain Chain: M
Molecule details ›
Chain: M
Length: 211 amino acids
Theoretical weight: 22.85 KDa
Source organism: Mus musculus
Expression system: Mus musculus
1C06 Fab light chain Chain: N
Molecule details ›
Chain: N
Length: 207 amino acids
Theoretical weight: 22.9 KDa
Source organism: Mus musculus
Expression system: Mus musculus
3H02 Fab heavy chain Chain: O
Molecule details ›
Chain: O
Length: 211 amino acids
Theoretical weight: 23.23 KDa
Source organism: Mus musculus
Expression system: Mus musculus
3H02 Fab light chain Chain: P
Molecule details ›
Chain: P
Length: 207 amino acids
Theoretical weight: 22.87 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand PKZ 3 x PKZ
Ligand AJP 14 x AJP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-23836
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

7 review citations

Update and nomenclature proposal for mammalian lysophospholipid acyltransferases, which create membrane phospholipid diversity.
Valentine et al. (2022)
6 more

7 mentions without citation

Access and utilization of long chain fatty acyl-CoA by zDHHC protein acyltransferases.
Puthenveetil et al. (2022)
6 more