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Half integration complex of Cas4/Cas1/Cas2 with Cas4 still on the Non-PAM side

Electron Microscopy
5.8Å resolution
PDB entry 7mib coloured by chain, front view.
PDB entry 7mib coloured by chain, side view.
PDB entry 7mib coloured by chain, top view.
Source organism: Geobacter sulfurreducens PCA
Assembly composition:
hetero decamer (preferred)
Entry contents: 2 distinct polypeptide molecules
4 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion and CRISPR-associated endoribonuclease Cas2 and DNA
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Multimeric state: hetero decamer
Accessible surface area: 94769.07 Å2
Buried surface area: 27465.59 Å2
Dissociation area: 530.36 Å2
Dissociation energy (ΔGdiss): -0.94 kcal/mol
Dissociation entropy (TΔSdiss): 10.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-121635

Macromolecules

CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion
Chains: A, B, C, D
Length: 559 amino acids
Theoretical weight: 62.6 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli
UniProt:
  • Canonical: Q74H36 (Residues: 1-559; Coverage: 100%)
Gene names: GSU0057, cas4-cas1
Pfam:
InterPro:
CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion in PDB entry 7mib, assembly 1, front view.
CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion in PDB entry 7mib, assembly 1, side view.
CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion in PDB entry 7mib, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q74H36  
155950100150200250300350400450500550
 
200400
UniProt
Q74H36
Chains
Domains
Secondary structure
Interaction interfaces
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CRISPR-associated endoribonuclease Cas2
Chains: E, F
Length: 95 amino acids
Theoretical weight: 11.19 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli
UniProt:
  • Canonical: Q74H35 (Residues: 1-95; Coverage: 100%)
Gene names: GSU0058, cas2
Pfam: CRISPR associated protein Cas2
InterPro:
CRISPR-associated endoribonuclease Cas2 in PDB entry 7mib, assembly 1, front view.
CRISPR-associated endoribonuclease Cas2 in PDB entry 7mib, assembly 1, side view.
CRISPR-associated endoribonuclease Cas2 in PDB entry 7mib, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q74H35  
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DNA chain G
Name: DNA (31-MER)
Representative chains: G
Length: 31 nucleotides
Theoretical weight: 9.51 KDa
DNA (31-MER) in PDB entry 7mib, assembly 1, front view.
DNA (31-MER) in PDB entry 7mib, assembly 1, side view.
DNA (31-MER) in PDB entry 7mib, assembly 1, top view.
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DNA chain H
Name: DNA (64-MER)
Representative chains: H
Length: 64 nucleotides
Theoretical weight: 19.66 KDa
Rfam: CRISPR RNA direct repeat element
DNA (64-MER) in PDB entry 7mib, assembly 1, front view.
DNA (64-MER) in PDB entry 7mib, assembly 1, side view.
DNA (64-MER) in PDB entry 7mib, assembly 1, top view.
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DNA chain I
Name: DNA (5'-D(P*CP*GP*GP*AP*AP*AP*AP*GP*AP*GP*CP*C)-3')
Representative chains: I
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
DNA (5'-D(P*CP*GP*GP*AP*AP*AP*AP*GP*AP*GP*CP*C)-3') in PDB entry 7mib, assembly 1, front view.
DNA (5'-D(P*CP*GP*GP*AP*AP*AP*AP*GP*AP*GP*CP*C)-3') in PDB entry 7mib, assembly 1, side view.
DNA (5'-D(P*CP*GP*GP*AP*AP*AP*AP*GP*AP*GP*CP*C)-3') in PDB entry 7mib, assembly 1, top view.
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DNA chain J
Name: DNA (45-MER)
Representative chains: J
Length: 45 nucleotides
Theoretical weight: 13.86 KDa
DNA (45-MER) in PDB entry 7mib, assembly 1, front view.
DNA (45-MER) in PDB entry 7mib, assembly 1, side view.
DNA (45-MER) in PDB entry 7mib, assembly 1, top view.
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