Structure analysis

Mtb 70S with P and E site tRNAs

Electron Microscopy
2.71Å resolution
Assembly composition:
hetero 55-mer (preferred)
Entry contents: 49 distinct polypeptide molecules
5 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 55-mer
Accessible surface area: 815786.1 Å2
Buried surface area: 309273.45 Å2
Dissociation area: 1,664.02 Å2
Dissociation energy (ΔGdiss): 13.59 kcal/mol
Dissociation entropy (TΔSdiss): 14.63 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-235516

Macromolecules

Chain: 0
Length: 57 amino acids
Theoretical weight: 6.53 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH99 (Residues: 1-57; Coverage: 100%)
Gene names: Rv0979A, rpmF
Pfam: Ribosomal L32p protein family
InterPro:

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Chain: 2
Length: 47 amino acids
Theoretical weight: 5.64 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH93 (Residues: 1-47; Coverage: 100%)
Gene names: MTV028.15, Rv3924c, rpmH
Pfam: Ribosomal protein L34
InterPro:

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Chain: 4
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH89 (Residues: 1-37; Coverage: 100%)
Gene names: MTCY13E12.14c, Rv3461c, rpmJ
Pfam: Ribosomal protein L36
InterPro:

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Chain: 7
Length: 24 amino acids
Theoretical weight: 2.88 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: A0A0U0UFA0 (Residues: 6-29; Coverage: 83%)
Gene names: ERS007703_02732, ERS007739_03374

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Chain: E
Length: 223 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH85 (Residues: 1-223; Coverage: 100%)
Gene names: MTCY210.21, Rv0702, rplD
Pfam: Ribosomal protein L4/L1 family
InterPro:

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Chain: N
Length: 180 amino acids
Theoretical weight: 19.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHD3 (Residues: 1-180; Coverage: 100%)
Gene names: MTCY13E12.09c, Rv3456c, rplQ
Pfam: Ribosomal protein L17
InterPro:

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Chain: O
Length: 122 amino acids
Theoretical weight: 13.21 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHD1 (Residues: 1-122; Coverage: 100%)
Gene names: MTCY210.39, Rv0720, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
InterPro:

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Chain: Q
Length: 129 amino acids
Theoretical weight: 14.56 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHC5 (Residues: 1-129; Coverage: 100%)
Gene names: MTCY06H11.07, Rv1643, rplT
Pfam: Ribosomal protein L20
InterPro:

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Chain: W
Length: 86 amino acids
Theoretical weight: 8.99 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHB3 (Residues: 1-86; Coverage: 100%)
Gene names: MTCY428.05, Rv2441c, rpmA
Pfam: Ribosomal L27 protein
InterPro:

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Chain: Y
Length: 77 amino acids
Theoretical weight: 8.87 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHA7 (Residues: 1-77; Coverage: 100%)
Gene names: MTCY210.28, Rv0709, rpmC
Pfam: Ribosomal L29 protein
InterPro:

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Chain: h
Length: 132 amino acids
Theoretical weight: 14.43 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH27 (Residues: 1-132; Coverage: 100%)
Gene names: MTCY210.37, Rv0718, rpsH
Pfam: Ribosomal protein S8
InterPro:

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Chain: l
Length: 124 amino acids
Theoretical weight: 13.88 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH63 (Residues: 1-124; Coverage: 100%)
Gene names: MTV040.10, Rv0682, rps12, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: o
Length: 89 amino acids
Theoretical weight: 10.5 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH55 (Residues: 1-89; Coverage: 100%)
Gene names: MTV002.50c, Rv2785c, rpsO
Pfam: Ribosomal protein S15
InterPro:

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Chain: p
Length: 162 amino acids
Theoretical weight: 17.47 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH53 (Residues: 1-162; Coverage: 100%)
Gene names: MTCY274.41c, Rv2909c, rpsP
Pfam: Ribosomal protein S16
InterPro:

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Chain: r
Length: 84 amino acids
Theoretical weight: 9.57 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH49 (Residues: 1-84; Coverage: 100%)
Gene names: MTCY21D4.18, Rv0055, rpsR, rpsR1
Pfam: Ribosomal protein S18
InterPro:

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Chain: t
Length: 86 amino acids
Theoretical weight: 9.43 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH41 (Residues: 1-86; Coverage: 100%)
Gene names: MTCY253.08c, Rv2412, rpsT
Pfam: Ribosomal protein S20
InterPro:

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Name: 23S ribosomal RNA
Representative chains: A
Length: 3138 nucleotides
Theoretical weight: 1017.82 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: B
Length: 115 nucleotides
Theoretical weight: 37.1 KDa
Rfam: 5S ribosomal RNA

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Name: 16S ribosomal RNA
Representative chains: a
Length: 1537 nucleotides
Theoretical weight: 498.4 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: tRNA (Met)
Representative chains: x, y
Length: 77 nucleotides
Theoretical weight: 24.8 KDa
Rfam: tRNA

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Name: Messenger RNA
Representative chains: z
Length: 26 nucleotides
Theoretical weight: 8.47 KDa

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