› 7mwu

X-ray diffraction
1.69Å resolution

Structure of the E. coli PutA proline dehydrogenase domain (residues 86-630) complexed with cyclobutanecarboxylic acid

Released:
DOI: 10.2210/pdb7mwu/pdb
PDB entry 7mwu coloured by chain, front view.
PDB entry 7mwu coloured by chain, side view.
PDB entry 7mwu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure-affinity relationships of reversible proline analog inhibitors targeting proline dehydrogenase.
Bogner AN, Tanner JJ
Org Biomol Chem 20 895-905 (2022)
PMID: 35018940

Function and Biology Details

Reactions catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PutA Chain: A
Molecule details ›
Chain: A
Length: 551 amino acids
Theoretical weight: 61.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09546 (Residues: 86-630; Coverage: 41%)
Gene names: JW0999, b1014, poaA, putA
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand ZPM 1 x ZPM
Ligand PGE 1 x PGE
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 73.083Å b: 141.818Å c: 146.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.234
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Exogenous Substrates Prevent the Decline in the Cellular ATP Content of Primary Rat Astrocytes During Glucose Deprivation.
Harders et al. (2024)
2 more

1 mention without citation

Structure-affinity relationships of reversible proline analog inhibitors targeting proline dehydrogenase.
Bogner et al. (2022)