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Cryo-EM structure of the monomeric form of SARS-CoV-2 nsp10-nsp14 (E191A)-RNA complex

Electron Microscopy
3.4Å resolution
PDB entry 7n0c coloured by chain, front view.
PDB entry 7n0c coloured by chain, side view.
PDB entry 7n0c coloured by chain, top view.
Source organism: Severe acute respiratory syndrome coronavirus 2
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules
2 distinct RNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: SARS-CoV-2 NSP10-NSP16 2'-O-methyltransferase complex and RNA
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Multimeric state: hetero tetramer
Accessible surface area: 35898.18 Å2
Buried surface area: 8847.5 Å2
Dissociation area: 1,538.84 Å2
Dissociation energy (ΔGdiss): 27.38 kcal/mol
Dissociation entropy (TΔSdiss): 12.33 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-145068

Macromolecules

Non-structural protein 10
Chain: A
Length: 139 amino acids
Theoretical weight: 14.8 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DTD1 (Residues: 4254-4392; Coverage: 2%)
Gene names: 1a-1b, rep
Pfam: Coronavirus RNA synthesis protein NSP10
InterPro:
Non-structural protein 10 in PDB entry 7n0c, assembly 1, front view.
Non-structural protein 10 in PDB entry 7n0c, assembly 1, side view.
Non-structural protein 10 in PDB entry 7n0c, assembly 1, top view.
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Guanine-N7 methyltransferase nsp14
Chain: B
Length: 527 amino acids
Theoretical weight: 59.83 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DTD1 (Residues: 5926-6452; Coverage: 7%)
Gene names: 1a-1b, rep
Pfam: Coronavirus proofreading exoribonuclease
InterPro:
Guanine-N7 methyltransferase nsp14 in PDB entry 7n0c, assembly 1, front view.
Guanine-N7 methyltransferase nsp14 in PDB entry 7n0c, assembly 1, side view.
Guanine-N7 methyltransferase nsp14 in PDB entry 7n0c, assembly 1, top view.
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RNA chain T
Name: RNA (25-MER)
Representative chains: T
Length: 37 nucleotides
Theoretical weight: 11.9 KDa
RNA (25-MER) in PDB entry 7n0c, assembly 1, front view.
RNA (25-MER) in PDB entry 7n0c, assembly 1, side view.
RNA (25-MER) in PDB entry 7n0c, assembly 1, top view.
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RNA chain D
Name: RNA (24-MER)
Representative chains: D
Length: 33 nucleotides
Theoretical weight: 10.46 KDa
RNA (24-MER) in PDB entry 7n0c, assembly 1, front view.
RNA (24-MER) in PDB entry 7n0c, assembly 1, side view.
RNA (24-MER) in PDB entry 7n0c, assembly 1, top view.
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