› 7ncz

X-ray diffraction
1.64Å resolution

Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with hexa-mannuronic acid

Released:
DOI: 10.2210/pdb7ncz/pdb
PDB entry 7ncz coloured by chain, front view.
PDB entry 7ncz coloured by chain, side view.
PDB entry 7ncz coloured by chain, top view.
Source organism: Paradendryphiella salina
Entry authors: Fredslund F, Welner DH, Wilkens C

Function and Biology Details

Reaction catalysed: 
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alginate lyase 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 25.45 KDa
Source organism: Paradendryphiella salina
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A485PVH1 (Residues: 23-243; Coverage: 100%)
Gene name: PsAlg7A
Sequence domains: Alginate lyase

Ligands and Environments

Carbohydrate polymer : NEW Components: BEM
Carbohydrate polymer
1 bound ligand:
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P212121
Unit cell:
a: 34.81Å b: 80.9Å c: 81.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.19
Expression system: Komagataella pastoris

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