Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- DNA-directed DNA polymerase, family B
- DNA primase large subunit, eukaryotic/archaeal
- DNA primase, large subunit, eukaryotic
- DNA primase, small subunit, eukaryotic/archaeal
- DNA polymerase alpha, subunit B
- NSP1 globular domain superfamily, betacoronavirus
- NSP1, globular domain, betacoronavirus
- NSP1, globular domain, alpha/betacoronavirus
14 more domains
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140800 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA polymerase alpha catalytic subunit
Molecule details ›
Chain: A
Length: 1170 amino acids
Theoretical weight: 133.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains:
Length: 1170 amino acids
Theoretical weight: 133.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
- Canonical:
P09884 (Residues: 334-1462; Coverage: 77%)
Sequence domains:
DNA polymerase alpha subunit B
Molecule details ›
Chain: B
Length: 452 amino acids
Theoretical weight: 49.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains:
Length: 452 amino acids
Theoretical weight: 49.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
- Canonical: Q14181 (Residues: 149-598; Coverage: 75%)
Sequence domains:
DNA primase small subunit
Molecule details ›
Chain: C
Length: 441 amino acids
Theoretical weight: 52.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains: DNA primase small subunit
Length: 441 amino acids
Theoretical weight: 52.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
- Canonical: P49642 (Residues: 1-420; Coverage: 100%)
Sequence domains: DNA primase small subunit
DNA primase large subunit
Molecule details ›
Chain: D
Length: 509 amino acids
Theoretical weight: 58.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
Length: 509 amino acids
Theoretical weight: 58.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
- Canonical: P49643 (Residues: 1-509; Coverage: 100%)
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
Host translation inhibitor nsp1
Molecule details ›
Chain: E
Length: 119 amino acids
Theoretical weight: 12.96 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Escherichia coli
UniProt:
Sequence domains: Betacoronavirus replicase NSP1
Length: 119 amino acids
Theoretical weight: 12.96 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Escherichia coli
UniProt:
- Canonical: P0C6U8 (Residues: 13-127; Coverage: 3%)
Sequence domains: Betacoronavirus replicase NSP1
