› 7or6

X-ray diffraction
2.12Å resolution

The crystal structure of the domain-swapped dimer of onconase

Released:
DOI: 10.2210/pdb7or6/pdb
PDB entry 7or6 coloured by chain, front view.
PDB entry 7or6 coloured by chain, side view.
PDB entry 7or6 coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
The crystal structure of the domain-swapped dimer of onconase highlights some catalytic and antitumor activity features of the enzyme.
Gotte G, Campagnari R, Loreto D, Bettin I, Calzetti F, Menegazzi M, Merlino A
Int J Biol Macromol 191 560-571 (2021)
PMID: 34563576

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein P-30 Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 105 amino acids
Theoretical weight: 11.97 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22069 (Residues: 2-104; Coverage: 99%)
Sequence domains: Pancreatic ribonuclease

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: P212121
Unit cell:
a: 28.29Å b: 60.05Å c: 101.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Role of the Ribonuclease ONCONASE in miRNA Biogenesis and tRNA Processing: Focus on Cancer and Viral Infections.
Menegazzi et al. (2022)
4 other articles