7owg

Electron Microscopy
4.7Å resolution

human DEPTOR in a complex with mutant human mTORC1 A1459P

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-154636 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein kinase mTOR Chain: B
Molecule details ›
Chain: B
Length: 2549 amino acids
Theoretical weight: 287.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42345 (Residues: 1-2549; Coverage: 100%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chain: E
Molecule details ›
Chain: E
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
DEP domain-containing mTOR-interacting protein Chain: O
Molecule details ›
Chain: O
Length: 409 amino acids
Theoretical weight: 46.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TB45 (Residues: 1-409; Coverage: 100%)
Gene names: DEPDC6, DEPTOR
Sequence domains: Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)
Regulatory-associated protein of mTOR Chain: Y
Molecule details ›
Chain: Y
Length: 1335 amino acids
Theoretical weight: 149.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N122 (Residues: 1-1335; Coverage: 100%)
Gene names: KIAA1303, RAPTOR, RPTOR
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.7Å
Relevant EMDB volumes: EMD-13097
Expression systems:
  • Homo sapiens
  • Escherichia coli