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Structure of KDNase from Trichophyton Rubrum in complex with 2-keto-3-deoxynononic acid

X-ray diffraction
0.99Å resolution
PDB entry 7p1u coloured by chain, front view.
PDB entry 7p1u coloured by chain, side view.
PDB entry 7p1u coloured by chain, top view.
Source organism: Trichophyton rubrum
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Sialidase domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 14354.21 Å2
Buried surface area: 237.55 Å2
Dissociation area: 118.77 Å2
Dissociation energy (ΔGdiss): 1.02 kcal/mol
Dissociation entropy (TΔSdiss): 0.85 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-103140
Assembly 2
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Assembly Name: Sialidase domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 14365.35 Å2
Buried surface area: 245.9 Å2
Dissociation area: 122.95 Å2
Dissociation energy (ΔGdiss): 0.32 kcal/mol
Dissociation entropy (TΔSdiss): 0.85 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-103140

Macromolecules

Sialidase domain-containing protein
Chains: A, B
Length: 386 amino acids
Theoretical weight: 42.21 KDa
Source organism: Trichophyton rubrum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A178EUH2 (Residues: 23-408; Coverage: 100%)
Gene name: A7C99_5399
Pfam: BNR repeat-like domain
InterPro:
Sialidase domain-containing protein in PDB entry 7p1u, assembly 1, front view.
Sialidase domain-containing protein in PDB entry 7p1u, assembly 1, side view.
Sialidase domain-containing protein in PDB entry 7p1u, assembly 1, top view.
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