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Complex I from E. coli, DDM/LMNG-purified, with DQ, Open state

Electron Microscopy
2.7Å resolution
PDB entry 7p7j coloured by chain, front view.
PDB entry 7p7j coloured by chain, side view.
PDB entry 7p7j coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Assembly composition:
hetero tridecamer (preferred)
Entry contents: 13 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: NADH-quinone oxidoreductase
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Multimeric state: hetero tridecamer
Accessible surface area: 137246.46 Å2
Buried surface area: 96624.0 Å2
Dissociation area: 4,340 Å2
Dissociation energy (ΔGdiss): 74.23 kcal/mol
Dissociation entropy (TΔSdiss): 18.05 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-142708

Macromolecules

NADH-quinone oxidoreductase subunit F
Chain: F
Length: 439 amino acids
Theoretical weight: 48.51 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31979 (Residues: 4-442; Coverage: 99%)
Gene names: JW2279, b2284, nuoF
Pfam:
InterPro:
NADH-quinone oxidoreductase subunit F in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit F in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit F in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit E
Chain: E
Length: 156 amino acids
Theoretical weight: 17.39 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFD1 (Residues: 11-166; Coverage: 94%)
Gene names: JW2280, b2285, nuoE
Pfam: Thioredoxin-like [2Fe-2S] ferredoxin
InterPro:
NADH-quinone oxidoreductase subunit E in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit E in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit E in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit G
Chain: G
Length: 905 amino acids
Theoretical weight: 99.96 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33602 (Residues: 2-906; Coverage: 100%)
Gene names: JW2278, b2283, nuoG
Pfam:
InterPro:
NADH-quinone oxidoreductase subunit G in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit G in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit G in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit C/D
Chain: C
Length: 600 amino acids
Theoretical weight: 68.81 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33599 (Residues: 1-596; Coverage: 100%)
Gene names: JW5375, b2286, nuoC, nuoCD, nuoD
Pfam:
InterPro:
NADH-quinone oxidoreductase subunit C/D in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit C/D in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit C/D in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit B
Chain: B
Length: 220 amino acids
Theoretical weight: 25.08 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFC7 (Residues: 1-220; Coverage: 100%)
Gene names: JW5875, b2287, nuoB
Pfam: NADH ubiquinone oxidoreductase, 20 Kd subunit
InterPro:
NADH-quinone oxidoreductase subunit B in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit B in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit B in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit I
Chain: I
Length: 180 amino acids
Theoretical weight: 20.56 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFD6 (Residues: 1-180; Coverage: 100%)
Gene names: JW2276, b2281, nuoI
Pfam: 4Fe-4S binding domain
InterPro:
NADH-quinone oxidoreductase subunit I in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit I in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit I in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit H
Chain: H
Length: 325 amino acids
Theoretical weight: 36.24 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFD4 (Residues: 1-325; Coverage: 100%)
Gene names: JW2277, b2282, nuoH
Pfam: NADH dehydrogenase
InterPro:
NADH-quinone oxidoreductase subunit H in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit H in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit H in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit A
Chain: A
Length: 147 amino acids
Theoretical weight: 16.47 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFC3 (Residues: 1-147; Coverage: 100%)
Gene names: JW2283, b2288, nuoA
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
InterPro:
NADH-quinone oxidoreductase subunit A in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit A in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit A in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit L
Chain: L
Length: 613 amino acids
Theoretical weight: 66.51 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33607 (Residues: 1-613; Coverage: 100%)
Gene names: JW2273, b2278, nuoL
Pfam:
InterPro:
NADH-quinone oxidoreductase subunit L in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit L in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit L in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit M
Chain: M
Length: 504 amino acids
Theoretical weight: 56 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFE8 (Residues: 1-504; Coverage: 99%)
Gene names: JW2272, b2277, nuoM
Pfam: Proton-conducting membrane transporter
InterPro:
NADH-quinone oxidoreductase subunit M in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit M in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit M in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit N
Chain: N
Length: 485 amino acids
Theoretical weight: 52.07 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFF0 (Residues: 1-485; Coverage: 100%)
Gene names: JW2271, b2276, nuoN
Pfam: Proton-conducting membrane transporter
InterPro:
NADH-quinone oxidoreductase subunit N in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit N in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit N in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit K
Chain: K
Length: 100 amino acids
Theoretical weight: 10.85 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFE4 (Residues: 1-100; Coverage: 100%)
Gene names: JW2274, b2279, nuoK
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
InterPro:
NADH-quinone oxidoreductase subunit K in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit K in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit K in PDB entry 7p7j, assembly 1, top view.
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NADH-quinone oxidoreductase subunit J
Chain: J
Length: 184 amino acids
Theoretical weight: 19.89 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFE0 (Residues: 1-184; Coverage: 100%)
Gene names: JW2275, b2280, nuoJ
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
InterPro:
NADH-quinone oxidoreductase subunit J in PDB entry 7p7j, assembly 1, front view.
NADH-quinone oxidoreductase subunit J in PDB entry 7p7j, assembly 1, side view.
NADH-quinone oxidoreductase subunit J in PDB entry 7p7j, assembly 1, top view.
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