Function and Biology Details
Reaction catalysed:
2 H(2) + NAD(+) + 2 oxidized ferredoxin = 5 H(+) + NADH + 2 reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- 4Fe-4S ferredoxin-type, iron-sulphur binding domain
- Thioredoxin-like superfamily
- 4Fe-4S ferredoxin, iron-sulphur binding, conserved site
- NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding
- NADH-quinone oxidoreductase subunit E-like
- NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain
- NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain superfamily
- NADH-ubiquinone oxidoreductase 51kDa subunit, FMN-binding domain superfamily
11 more domains
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
Bifurcating [FeFe] hydrogenase (preferred)
PDBe Complex ID:
PDB-CPX-520497 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Bifurcating [FeFe] hydrogenase alpha subunit
Molecule details ›
Chains: A, a
Length: 645 amino acids
Theoretical weight: 72.35 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
Sequence domains:
Length: 645 amino acids
Theoretical weight: 72.35 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
- Canonical:
O52683 (Residues: 1-645; Coverage: 100%)
Sequence domains:
Bifurcating [FeFe] hydrogenase beta subunit
Molecule details ›
Chains: B, b
Length: 626 amino acids
Theoretical weight: 68.77 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
Sequence domains:
Length: 626 amino acids
Theoretical weight: 68.77 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
- Canonical: O52682 (Residues: 1-626; Coverage: 100%)
Sequence domains:
Bifurcating [FeFe] hydrogenase gamma subunit
Molecule details ›
Chains: C, c
Length: 189 amino acids
Theoretical weight: 20.94 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
Length: 189 amino acids
Theoretical weight: 20.94 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
- Canonical: O52681 (Residues: 1-161; Coverage: 100%)
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
