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Core human replisome

Electron Microscopy
3.2Å resolution
PDB entry 7pfo coloured by chain, front view.
PDB entry 7pfo coloured by chain, side view.
PDB entry 7pfo coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero 21-mer (preferred)
Entry contents: 17 distinct polypeptide molecules
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: GINS complex and DNA
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Multimeric state: hetero 21-mer
Accessible surface area: 342497.19 Å2
Buried surface area: 127621.42 Å2
Dissociation area: 1,094.49 Å2
Dissociation energy (ΔGdiss): -5.57 kcal/mol
Dissociation entropy (TΔSdiss): 16.99 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-232356

Macromolecules

DNA replication licensing factor MCM2
Chain: 2
Length: 904 amino acids
Theoretical weight: 102.03 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49736 (Residues: 1-904; Coverage: 100%)
Gene names: BM28, CCNL1, CDCL1, KIAA0030, MCM2
Pfam:
InterPro:
DNA replication licensing factor MCM2 in PDB entry 7pfo, assembly 1, front view.
DNA replication licensing factor MCM2 in PDB entry 7pfo, assembly 1, side view.
DNA replication licensing factor MCM2 in PDB entry 7pfo, assembly 1, top view.
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DNA replication licensing factor MCM3
Chain: 3
Length: 808 amino acids
Theoretical weight: 91.11 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25205 (Residues: 1-808; Coverage: 100%)
Gene name: MCM3
Pfam:
InterPro:
DNA replication licensing factor MCM3 in PDB entry 7pfo, assembly 1, front view.
DNA replication licensing factor MCM3 in PDB entry 7pfo, assembly 1, side view.
DNA replication licensing factor MCM3 in PDB entry 7pfo, assembly 1, top view.
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DNA replication licensing factor MCM4
Chain: 4
Length: 863 amino acids
Theoretical weight: 96.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P33991 (Residues: 1-863; Coverage: 100%)
Gene names: CDC21, MCM4
Pfam:
InterPro:
DNA replication licensing factor MCM4 in PDB entry 7pfo, assembly 1, front view.
DNA replication licensing factor MCM4 in PDB entry 7pfo, assembly 1, side view.
DNA replication licensing factor MCM4 in PDB entry 7pfo, assembly 1, top view.
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DNA replication licensing factor MCM5
Chain: 5
Length: 734 amino acids
Theoretical weight: 82.41 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P33992 (Residues: 1-734; Coverage: 100%)
Gene names: CDC46, MCM5
Pfam:
InterPro:
DNA replication licensing factor MCM5 in PDB entry 7pfo, assembly 1, front view.
DNA replication licensing factor MCM5 in PDB entry 7pfo, assembly 1, side view.
DNA replication licensing factor MCM5 in PDB entry 7pfo, assembly 1, top view.
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DNA replication licensing factor MCM6
Chain: 6
Length: 821 amino acids
Theoretical weight: 93.01 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14566 (Residues: 1-821; Coverage: 100%)
Gene name: MCM6
Pfam:
InterPro:
DNA replication licensing factor MCM6 in PDB entry 7pfo, assembly 1, front view.
DNA replication licensing factor MCM6 in PDB entry 7pfo, assembly 1, side view.
DNA replication licensing factor MCM6 in PDB entry 7pfo, assembly 1, top view.
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DNA replication licensing factor MCM7
Chain: 7
Length: 719 amino acids
Theoretical weight: 81.41 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P33993 (Residues: 1-719; Coverage: 100%)
Gene names: CDC47, MCM2, MCM7
Pfam:
InterPro:
DNA replication licensing factor MCM7 in PDB entry 7pfo, assembly 1, front view.
DNA replication licensing factor MCM7 in PDB entry 7pfo, assembly 1, side view.
DNA replication licensing factor MCM7 in PDB entry 7pfo, assembly 1, top view.
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DNA polymerase epsilon subunit 2
Chain: A
Length: 527 amino acids
Theoretical weight: 59.6 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P56282 (Residues: 1-527; Coverage: 100%)
Gene names: DPE2, POLE2
Pfam:
InterPro:
DNA polymerase epsilon subunit 2 in PDB entry 7pfo, assembly 1, front view.
DNA polymerase epsilon subunit 2 in PDB entry 7pfo, assembly 1, side view.
DNA polymerase epsilon subunit 2 in PDB entry 7pfo, assembly 1, top view.
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DNA polymerase epsilon catalytic subunit A
Chain: B
Length: 2286 amino acids
Theoretical weight: 261.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q07864 (Residues: 1-2286; Coverage: 100%)
Gene names: POLE, POLE1
Pfam:
InterPro:
DNA polymerase epsilon catalytic subunit A in PDB entry 7pfo, assembly 1, front view.
DNA polymerase epsilon catalytic subunit A in PDB entry 7pfo, assembly 1, side view.
DNA polymerase epsilon catalytic subunit A in PDB entry 7pfo, assembly 1, top view.
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Cell division control protein 45 homolog
Chain: C
Length: 569 amino acids
Theoretical weight: 66.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75419 (Residues: 1-160, 165-566; Coverage: 99%)
Gene names: CDC45, CDC45L, CDC45L2, UNQ374/PRO710
Pfam: CDC45-like protein
InterPro: CDC45 family
Cell division control protein 45 homolog in PDB entry 7pfo, assembly 1, front view.
Cell division control protein 45 homolog in PDB entry 7pfo, assembly 1, side view.
Cell division control protein 45 homolog in PDB entry 7pfo, assembly 1, top view.
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DNA replication complex GINS protein PSF1
Chain: D
Length: 196 amino acids
Theoretical weight: 23.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14691 (Residues: 1-196; Coverage: 100%)
Gene names: GINS1, KIAA0186, PSF1
Pfam: GINS complex protein helical bundle domain
InterPro:
DNA replication complex GINS protein PSF1 in PDB entry 7pfo, assembly 1, front view.
DNA replication complex GINS protein PSF1 in PDB entry 7pfo, assembly 1, side view.
DNA replication complex GINS protein PSF1 in PDB entry 7pfo, assembly 1, top view.
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DNA replication complex GINS protein PSF2
Chain: E
Length: 185 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9Y248 (Residues: 1-185; Coverage: 100%)
Gene names: CGI-122, DC5, GINS2, HSPC037, PSF2
Pfam: GINS complex protein helical bundle domain
InterPro:
DNA replication complex GINS protein PSF2 in PDB entry 7pfo, assembly 1, front view.
DNA replication complex GINS protein PSF2 in PDB entry 7pfo, assembly 1, side view.
DNA replication complex GINS protein PSF2 in PDB entry 7pfo, assembly 1, top view.
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DNA replication complex GINS protein PSF3
Chain: F
Length: 216 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BRX5 (Residues: 1-216; Coverage: 100%)
Gene names: GINS3, PSF3
Pfam:
InterPro:
DNA replication complex GINS protein PSF3 in PDB entry 7pfo, assembly 1, front view.
DNA replication complex GINS protein PSF3 in PDB entry 7pfo, assembly 1, side view.
DNA replication complex GINS protein PSF3 in PDB entry 7pfo, assembly 1, top view.
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DNA replication complex GINS protein SLD5
Chain: G
Length: 262 amino acids
Theoretical weight: 30.11 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BRT9 (Residues: 1-223; Coverage: 100%)
Gene names: GINS4, SLD5
Pfam:
InterPro:
DNA replication complex GINS protein SLD5 in PDB entry 7pfo, assembly 1, front view.
DNA replication complex GINS protein SLD5 in PDB entry 7pfo, assembly 1, side view.
DNA replication complex GINS protein SLD5 in PDB entry 7pfo, assembly 1, top view.
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WD repeat and HMG-box DNA-binding protein 1
Chains: H, I, J
Length: 1161 amino acids
Theoretical weight: 130.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75717 (Residues: 1-1129; Coverage: 100%)
Gene names: AND1, WDHD1
Pfam:
InterPro:
WD repeat and HMG-box DNA-binding protein 1 in PDB entry 7pfo, assembly 1, front view.
WD repeat and HMG-box DNA-binding protein 1 in PDB entry 7pfo, assembly 1, side view.
WD repeat and HMG-box DNA-binding protein 1 in PDB entry 7pfo, assembly 1, top view.
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Protein timeless homolog
Chain: K
Length: 1209 amino acids
Theoretical weight: 138.9 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UNS1 (Residues: 1-1208; Coverage: 100%)
Gene names: TIM, TIM1, TIMELESS, TIMELESS1
Pfam:
InterPro:
Protein timeless homolog in PDB entry 7pfo, assembly 1, front view.
Protein timeless homolog in PDB entry 7pfo, assembly 1, side view.
Protein timeless homolog in PDB entry 7pfo, assembly 1, top view.
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TIMELESS-interacting protein
Chain: L
Length: 301 amino acids
Theoretical weight: 34.6 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BVW5 (Residues: 1-301; Coverage: 100%)
Gene name: TIPIN
Pfam: Replication Fork Protection Component Swi3
InterPro: Chromosome segregation in meiosis protein 3
TIMELESS-interacting protein in PDB entry 7pfo, assembly 1, front view.
TIMELESS-interacting protein in PDB entry 7pfo, assembly 1, side view.
TIMELESS-interacting protein in PDB entry 7pfo, assembly 1, top view.
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Claspin
Chain: Q
Length: 1371 amino acids
Theoretical weight: 155.18 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9HAW4 (Residues: 1-1339; Coverage: 100%)
Gene name: CLSPN
InterPro: Claspin
Claspin in PDB entry 7pfo, assembly 1, front view.
Claspin in PDB entry 7pfo, assembly 1, side view.
Claspin in PDB entry 7pfo, assembly 1, top view.
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DNA chain M
Name: Leading strand DNA
Representative chains: M
Length: 85 nucleotides
Theoretical weight: 26.4 KDa
Leading strand DNA in PDB entry 7pfo, assembly 1, front view.
Leading strand DNA in PDB entry 7pfo, assembly 1, side view.
Leading strand DNA in PDB entry 7pfo, assembly 1, top view.
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DNA chain N
Name: Lagging Strand DNA
Representative chains: N
Length: 54 nucleotides
Theoretical weight: 16.41 KDa
Lagging Strand DNA in PDB entry 7pfo, assembly 1, front view.
Lagging Strand DNA in PDB entry 7pfo, assembly 1, side view.
Lagging Strand DNA in PDB entry 7pfo, assembly 1, top view.
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