Structure analysis

Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP-Pi)

Electron Microscopy
3.1Å resolution
Source organism: Escherichia coli K-12
Assembly composition:
hetero 59-mer (preferred)
Entry contents: 53 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 59-mer
Accessible surface area: 770266.8 Å2
Buried surface area: 338052.82 Å2
Dissociation area: 952.75 Å2
Dissociation energy (ΔGdiss): -1.29 kcal/mol
Dissociation entropy (TΔSdiss): 13.44 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135494

Macromolecules

Chain: 0
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7N4 (Residues: 1-57; Coverage: 100%)
Pfam: Ribosomal L32p protein family
InterPro:

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Chain: 2
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Pfam: Ribosomal protein L34
InterPro:

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Chain: 4
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Pfam: Ribosomal protein L36
InterPro:

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Chain: E
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Pfam: Ribosomal protein L4/L1 family
InterPro:

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Chain: N
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0AG44 (Residues: 1-127; Coverage: 100%)
Pfam: Ribosomal protein L17
InterPro:

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Chain: O
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0C018 (Residues: 1-117; Coverage: 100%)
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
InterPro:

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Chain: Q
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7L3 (Residues: 1-118; Coverage: 100%)
Pfam: Ribosomal protein L20
InterPro:

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Chain: W
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)
Pfam: Ribosomal L27 protein
InterPro:

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Chain: Y
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Pfam: Ribosomal L29 protein
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Chain: h
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Pfam: Ribosomal protein S8
InterPro:

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Chain: l
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: n
Length: 102 amino acids
Theoretical weight: 11.68 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0AG59 (Residues: 1-35, 36-101; Coverage: 100%)
Pfam: Ribosomal protein S14p/S29e
InterPro:

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Chain: o
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Pfam: Ribosomal protein S15
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Chain: p
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Pfam: Ribosomal protein S16
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Chain: t
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Pfam: Ribosomal protein S20
InterPro:

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Chain: u
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Pfam: Ribosomal protein S21
InterPro:

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Name: 23S ribosomal RNA
Representative chains: A
Source organism: Escherichia coli K-12 [83333]
Length: 2903 nucleotides
Theoretical weight: 941.54 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: B
Source organism: Escherichia coli K-12 [83333]
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Rfam: 5S ribosomal RNA

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Name: 16S ribosomal RNA
Representative chains: a
Source organism: Escherichia coli K-12 [83333]
Length: 1542 nucleotides
Theoretical weight: 499.87 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: P-site tRNA(fMet)
Representative chains: v
Source organism: Escherichia coli K-12 [83333]
Length: 77 nucleotides
Theoretical weight: 24.82 KDa
Rfam: tRNA

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Name: P-site fMet-Phe-tRNA(Phe)
Representative chains: w
Source organism: Escherichia coli K-12 [83333]
Length: 76 nucleotides
Theoretical weight: 24.64 KDa
Rfam: tRNA

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Name: Messenger RNA
Representative chains: z
Source organism: Escherichia coli K-12 [83333]
Length: 33 nucleotides
Theoretical weight: 10.48 KDa

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