› 7q1v

Electron Microscopy
6.18Å resolution

Arches protomer (trimer of TrwG/VirB8peri) structure from the fully-assembled R388 type IV secretion system determined by cryo-EM.

Released:
DOI: 10.2210/pdb7q1v/pdb
PDB entry 7q1v coloured by chain, front view.
PDB entry 7q1v coloured by chain, side view.
PDB entry 7q1v coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cryo-EM structure of a type IV secretion system.
Macé K, Vadakkepat AK, Redzej A, Lukoyanova N, Oomen C, Braun N, Ukleja M, Lu F, Costa TRD, Orlova EV, Baker D, Cong Q, Waksman G
OpenAccess logo Nature 607 191-196 (2022)
PMID: 35732732
Related structures: EMD-13767

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial virulence protein VirB8 domain-containing protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 231 amino acids
Theoretical weight: 25.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O50335 (Residues: 1-231; Coverage: 100%)
Gene name: trwG
Sequence domains: VirB8 protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.18Å
Relevant EMDB volumes: EMD-13767
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structural and functional diversity of type IV secretion systems.
Costa et al. (2024)
7 more

1 mention without citation

Primary architecture and energy requirements of Type III and Type IV secretion systems.
Cabezón et al. (2023)