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Cryo-EM structure of a type IV secretion system.

<div class='caption-title'>Overall structure of the R388 conjugative T4SS.</div>
<div class='caption-title'>Molecular details of IMC protein structures and interactions.</div>
<div class='caption-title'>Molecular details of stalk and arches protein structures and interactions, and structure validation by co-evolution analysis.</div>
Macé K, Vadakkepat AK, Redzej A, Lukoyanova N, Oomen C, Braun N, Ukleja M, Lu F, Costa TRD, Orlova EV, Baker D, Cong Q, Waksman G
OpenAccess logo Nature 607 191-196 (2022)
Related entries: 7o3j, 7o3t, 7o3v, 7o41, 7o42, 7o43, 7oiu

Cited: 32 times
EuropePMC logo PMID: 35732732

Abstract

Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell1. It is the primary means by which antibiotic resistance genes spread among bacterial populations2,3. In Gram-negative bacteria, conjugation is mediated by a large transport apparatus-the conjugative type IV secretion system (T4SS)-produced by the donor cell and embedded in both its outer and inner membranes. The T4SS also elaborates a long extracellular filament-the conjugative pilus-that is essential for DNA transfer4,5. Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components involved in pilus biogenesis. We added the two remaining components to the structural model using co-evolution analysis of protein interfaces, to enable the reconstitution of the entire system including the pilus. This structure describes the exceptionally large protein-protein interaction network required to assemble the many components that constitute a T4SS and provides insights on the unique mechanism by which they elaborate pili.

Reviews - 7q1v mentioned but not cited (1)

  1. Primary architecture and energy requirements of Type III and Type IV secretion systems. Cabezón E, Valenzuela-Gómez F, Arechaga I. Front Cell Infect Microbiol 13 1255852 (2023)


Reviews citing this publication (8)

  1. Structural and functional diversity of type IV secretion systems. Costa TRD, Patkowski JB, Macé K, Christie PJ, Waksman G. Nat Rev Microbiol 22 170-185 (2024)
  2. The multifaceted virulence of adherent-invasive Escherichia coli. Mansour S, Asrar T, Elhenawy W. Gut Microbes 15 2172669 (2023)
  3. Recruitment of heterologous substrates by bacterial secretion systems for transkingdom translocation. Guzmán-Herrador DL, Fernández-Gómez A, Llosa M. Front Cell Infect Microbiol 13 1146000 (2023)
  4. DNA Transport through the Dynamic Type IV Secretion System. Ryan ME, Damke PP, Shaffer CL. Infect Immun 91 e0043622 (2023)
  5. Plasmids pick a bacterial partner before committing to conjugation. Frankel G, David S, Low WW, Seddon C, Wong JLC, Beis K. Nucleic Acids Res 51 8925-8933 (2023)
  6. The Helicobacter pylori cag pathogenicity island as a determinant of gastric cancer risk. Tran SC, Bryant KN, Cover TL. Gut Microbes 16 2314201 (2024)
  7. Comparative Analysis of T4SS Molecular Architectures. Zehra M, Heo J, Chung JM, Durie CL. J Microbiol Biotechnol 33 1543-1551 (2023)
  8. Structural Analysis of Protein Complexes by Cryo-Electron Microscopy. Ignatiou A, Macé K, Redzej A, Costa TRD, Waksman G, Orlova EV. Methods Mol Biol 2715 431-470 (2024)

Articles citing this publication (23)

  1. DPAM: A domain parser for AlphaFold models. Zhang J, Schaeffer RD, Durham J, Cong Q, Grishin NV. Protein Sci 32 e4548 (2023)
  2. Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer. Couturier A, Virolle C, Goldlust K, Berne-Dedieu A, Reuter A, Nolivos S, Yamaichi Y, Bigot S, Lesterlin C. Nat Commun 14 294 (2023)
  3. Cryo-EM structure of the Agrobacterium tumefaciens T4SS-associated T-pilus reveals stoichiometric protein-phospholipid assembly. Kreida S, Narita A, Johnson MD, Tocheva EI, Das A, Ghosal D, Jensen GJ. Structure 31 385-394.e4 (2023)
  4. Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly. Conners R, León-Quezada RI, McLaren M, Bennett NJ, Daum B, Rakonjac J, Gold VAM. Nat Commun 14 2724 (2023)
  5. Mechanism of regulation of the Helicobacter pylori Cagβ ATPase by CagZ. Wu X, Zhao Y, Zhang H, Yang W, Yang J, Sun L, Jiang M, Wang Q, Wang Q, Ye X, Zhang X, Wu Y. Nat Commun 14 479 (2023)
  6. IncFV plasmid pED208: Sequence analysis and evidence for translocation of maintenance/leading region proteins through diverse type IV secretion systems. Al Mamun AAM, Kissoon K, Kishida K, Shropshire WC, Hanson B, Christie PJ. Plasmid 123-124 102652 (2022)
  7. Role of the CagY antenna projection in Helicobacter pylori Cag type IV secretion system activity. Tran SC, McClain MS, Cover TL. Infect Immun 91 e0015023 (2023)
  8. Assembly mechanism of a Tad secretion system secretin-pilotin complex. Tassinari M, Rudzite M, Filloux A, Low HH. Nat Commun 14 5643 (2023)
  9. Emergence and Transfer of Plasmid-Harbored rmtB in a Clinical Multidrug-Resistant Pseudomonas aeruginosa Strain. Gao J, Wei X, Yin L, Jin Y, Bai F, Cheng Z, Wu W. Microorganisms 10 1818 (2022)
  10. Removal of Pseudomonas type IV pili by a small RNA virus. Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L. Science 384 eadl0635 (2024)
  11. Chimeric systems composed of swapped Tra subunits between distantly-related F plasmids reveal striking plasticity among type IV secretion machines. Kishida K, Li YG, Ogawa-Kishida N, Khara P, Al Mamun AAM, Bosserman RE, Christie PJ. PLoS Genet 20 e1011088 (2024)
  12. Functional characterization of VirB/VirD4 and Icm/Dot type IV secretion systems from the plant-pathogenic bacterium Xanthomonas euvesicatoria. Drehkopf S, Scheibner F, Büttner D. Front Cell Infect Microbiol 13 1203159 (2023)
  13. Growth in a biofilm promotes conjugation of a bla NDM-1-bearing plasmid between Klebsiella pneumoniae strains. Element SJ, Moran RA, Beattie E, Hall RJ, van Schaik W, Buckner MMC. mSphere 8 e0017023 (2023)
  14. Nanopore sensing reveals a preferential pathway for the co-translocational unfolding of a conjugative relaxase-DNA complex. Valenzuela-Gómez F, Arechaga I, Cabezón E. Nucleic Acids Res 51 6857-6869 (2023)
  15. The mating pilus of E. coli pED208 acts as a conduit for ssDNA during horizontal gene transfer. Beltrán L, Torsilieri H, Patkowski JB, Yang JE, Casanova J, Costa TRD, Wright ER, Egelman EH. mBio 15 e0285723 (2024)
  16. Characterization of ConE, the VirB4 Homolog of the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis. Murthy AC, Aleksanyan N, Morton GM, Toyoda HC, Kalashyan M, Chen S, Ragucci AE, Broulidakis MP, Swerdlow KJ, Bui MNN, Muccioli M, Berkmen MB. J Bacteriol 205 e0003323 (2023)
  17. Morphological remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography. Shepherd DC, Kaplan M, Vankadari N, Kim KW, Larson CL, Dutka P, Beare PA, Krzymowski E, Heinzen RA, Jensen GJ, Ghosal D. iScience 26 107210 (2023)
  18. Oligomerization and Adjuvant Activity of Peptides Derived from the VirB4-like ATPase of Clostridioides difficile. Sorokina J, Sokolova I, Majorina M, Ungur A, Troitskiy V, Tukhvatulin A, Melnik B, Belyi Y. Biomolecules 13 1012 (2023)
  19. PrgE: an OB-fold protein from plasmid pCF10 with striking differences to prototypical bacterial SSBs. Breidenstein A, Lamy A, Bader CP, Sun WS, Wanrooij PH, Berntsson RP. Life Sci Alliance 7 e202402693 (2024)
  20. Serine peptidase Vpr forms enzymatically active fibrils outside Bacillus bacteria revealed by cryo-EM. Cheng Y, Han J, Song M, Zhang S, Cao Q. Nat Commun 14 7503 (2023)
  21. Structural and functional characterization of TraI from pKM101 reveals basis for DNA processing. Breidenstein A, Ter Beek J, Berntsson RP. Life Sci Alliance 6 e202201775 (2023)
  22. Subdomains of the Helicobacter pylori Cag T4SS outer membrane core complex exhibit structural independence. Roberts JR, Tran SC, Frick-Cheng AE, Bryant KN, Okoye CD, McDonald WH, Cover TL, Ohi MD. Life Sci Alliance 7 e202302560 (2024)
  23. Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors. Oka GU, Souza DP, Sgro GG, Guzzo CR, Dunger G, Farah CS. EMBO Rep 25 1436-1452 (2024)


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