Structure analysis

Structure of the B. subtilis disome - collided 70S ribosome

Electron Microscopy
5.45Å resolution
Source organism: Bacillus subtilis
Assembly composition:
hetero 52-mer (preferred)
Entry contents: 47 distinct polypeptide molecules
4 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 52-mer
Accessible surface area: 720699.22 Å2
Buried surface area: 164790.08 Å2
Dissociation area: 910.55 Å2
Dissociation energy (ΔGdiss): 5.95 kcal/mol
Dissociation entropy (TΔSdiss): 10.95 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-281407

Macromolecules

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Chain: E
Length: 207 amino acids
Theoretical weight: 22.42 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P42921 (Residues: 1-207; Coverage: 100%)
Gene names: BSU01170, rplD
Pfam: Ribosomal protein L4/L1 family
InterPro:

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Chain: J
Length: 145 amino acids
Theoretical weight: 16.41 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P70974 (Residues: 1-145; Coverage: 100%)
Gene names: BSU01490, rplM
Pfam: Ribosomal protein L13
InterPro:

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Chain: N
Length: 120 amino acids
Theoretical weight: 13.77 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P20277 (Residues: 1-120; Coverage: 100%)
Gene names: BSU01440, rplQ
Pfam: Ribosomal protein L17
InterPro:

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Chain: O
Length: 120 amino acids
Theoretical weight: 12.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P46899 (Residues: 1-120; Coverage: 100%)
Gene names: BSU01320, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
InterPro:

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Chain: P
Length: 115 amino acids
Theoretical weight: 13.42 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: O31742 (Residues: 1-115; Coverage: 100%)
Gene names: BSU16040, rplS
Pfam: Ribosomal protein L19
InterPro:

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Chain: Q
Length: 119 amino acids
Theoretical weight: 13.67 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P55873 (Residues: 1-119; Coverage: 100%)
Gene names: BSU28850, rplT
Pfam: Ribosomal protein L20
InterPro:

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Chain: V
Length: 94 amino acids
Theoretical weight: 10.39 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P05657 (Residues: 1-94; Coverage: 100%)
Gene names: BSU27940, rpmA
Pfam: Ribosomal L27 protein
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Chain: Z
Length: 66 amino acids
Theoretical weight: 7.73 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12873 (Residues: 1-66; Coverage: 100%)
Gene names: BSU01240, rpmC
Pfam: Ribosomal L29 protein
InterPro:

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Chain: b
Length: 59 amino acids
Theoretical weight: 6.75 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: O34687 (Residues: 1-59; Coverage: 100%)
Gene names: BSU15080, rpmF
Pfam: Ribosomal L32p protein family
InterPro:

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Chain: d
Length: 44 amino acids
Theoretical weight: 5.27 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P05647 (Residues: 1-44; Coverage: 100%)
Gene names: BSU41060, rpmH
Pfam: Ribosomal protein L34
InterPro:

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Chain: f
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P20278 (Residues: 1-37; Coverage: 100%)
Gene names: BSU01400, rpmJ
Pfam: Ribosomal protein L36
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Chain: l
Length: 132 amino acids
Theoretical weight: 14.9 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12879 (Residues: 1-132; Coverage: 100%)
Gene names: BSU01300, rpsH
Pfam: Ribosomal protein S8
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Chain: n
Length: 102 amino acids
Theoretical weight: 11.69 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21471 (Residues: 1-102; Coverage: 100%)
Gene names: BSU01150, rpsJ, tetA
Pfam: Ribosomal protein S10p/S20e
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Chain: o
Length: 131 amino acids
Theoretical weight: 13.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P04969 (Residues: 1-131; Coverage: 100%)
Gene names: BSU01420, rpsK
Pfam: Ribosomal protein S11
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Chain: p
Length: 138 amino acids
Theoretical weight: 15.25 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21472 (Residues: 1-138; Coverage: 100%)
Gene names: BSU01100, fun, rpsL, strA
Pfam: Ribosomal protein S12/S23
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Chain: s
Length: 89 amino acids
Theoretical weight: 10.6 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21473 (Residues: 1-89; Coverage: 100%)
Gene names: BSU16680, rpsO
Pfam: Ribosomal protein S15
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Chain: t
Length: 90 amino acids
Theoretical weight: 10.15 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21474 (Residues: 1-90; Coverage: 100%)
Gene names: BSU15990, rpsP
Pfam: Ribosomal protein S16
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Chain: u
Length: 87 amino acids
Theoretical weight: 10.22 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12874 (Residues: 1-87; Coverage: 100%)
Gene names: BSU01250, rpsQ
Pfam: Ribosomal protein S17
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Chain: v
Length: 79 amino acids
Theoretical weight: 8.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21475 (Residues: 1-79; Coverage: 100%)
Gene names: BSU40890, rpsR
Pfam: Ribosomal protein S18
InterPro:

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Chain: w
Length: 92 amino acids
Theoretical weight: 10.61 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21476 (Residues: 1-92; Coverage: 100%)
Gene names: BSU01200, rpsS
Pfam: Ribosomal protein S19
InterPro:

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Chain: x
Length: 88 amino acids
Theoretical weight: 9.62 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21477 (Residues: 1-88; Coverage: 100%)
Gene names: BSU25550, rpsT, yqeO
Pfam: Ribosomal protein S20
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Name: 23S ribosomal RNA
Representative chains: A
Length: 2928 nucleotides
Theoretical weight: 949.61 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: B
Length: 119 nucleotides
Theoretical weight: 38.42 KDa
Rfam: 5S ribosomal RNA

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Name: 16S ribosomal RNA
Representative chains: W
Length: 1555 nucleotides
Theoretical weight: 503.7 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: tRNA
Representative chains: 1, y
Length: 77 nucleotides
Theoretical weight: 24.82 KDa
Rfam: tRNA

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