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Structure of the Rab GEF complex Mon1-Ccz1

Electron Microscopy
3.85Å resolution
PDB entry 7qla coloured by chain, front view.
PDB entry 7qla coloured by chain, side view.
PDB entry 7qla coloured by chain, top view.
Source organism: Thermochaetoides thermophila
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CCZ1/INTU/HSP4 first Longin domain-containing protein and Vacuolar fusion protein MON1
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Multimeric state: hetero dimer
Accessible surface area: 38964.61 Å2
Buried surface area: 3297.58 Å2
Dissociation area: 1,648.79 Å2
Dissociation energy (ΔGdiss): 12.79 kcal/mol
Dissociation entropy (TΔSdiss): 14.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124080

Macromolecules

Vacuolar fusion protein MON1
Chain: A
Length: 525 amino acids
Theoretical weight: 57.57 KDa
Source organism: Thermochaetoides thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SGS3 (Residues: 141-665; Coverage: 79%)
Gene name: CTHT_0067370
Pfam:
InterPro:
Vacuolar fusion protein MON1 in PDB entry 7qla, assembly 1, front view.
Vacuolar fusion protein MON1 in PDB entry 7qla, assembly 1, side view.
Vacuolar fusion protein MON1 in PDB entry 7qla, assembly 1, top view.
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CCZ1/INTU/HSP4 first Longin domain-containing protein
Chain: C
Length: 696 amino acids
Theoretical weight: 75.78 KDa
Source organism: Thermochaetoides thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SD94 (Residues: 1-796; Coverage: 87%)
Gene name: CTHT_0050970
Pfam: First Longin domain of INTU, CCZ1 and HPS4
InterPro:
CCZ1/INTU/HSP4 first Longin domain-containing protein in PDB entry 7qla, assembly 1, front view.
CCZ1/INTU/HSP4 first Longin domain-containing protein in PDB entry 7qla, assembly 1, side view.
CCZ1/INTU/HSP4 first Longin domain-containing protein in PDB entry 7qla, assembly 1, top view.
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