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Metazoan pre-targeting GET complex (cBUGG-in)

Electron Microscopy
3.3Å resolution
PDB entry 7ru9 coloured by chain, front view.
PDB entry 7ru9 coloured by chain, side view.
PDB entry 7ru9 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero octamer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero octamer
Accessible surface area: 66636.06 Å2
Buried surface area: 23762.75 Å2
Dissociation area: 3,533.07 Å2
Dissociation energy (ΔGdiss): 19.18 kcal/mol
Dissociation entropy (TΔSdiss): 30.1 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-145832

Macromolecules

ATPase GET3
Chains: A, B
Length: 343 amino acids
Theoretical weight: 38.57 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6IQE5 (Residues: 1-341; Coverage: 100%)
Gene names: asna1, get3, si:dkey-121b10.2, zgc:86799
Pfam: Anion-transporting ATPase
InterPro:
ATPase GET3 in PDB entry 7ru9, assembly 1, front view.
ATPase GET3 in PDB entry 7ru9, assembly 1, side view.
ATPase GET3 in PDB entry 7ru9, assembly 1, top view.
Molecule details ›

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Golgi to ER traffic protein 4 homolog
Chains: C, F
Length: 333 amino acids
Theoretical weight: 37.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7L5D6 (Residues: 1-327; Coverage: 100%)
Gene names: C7orf20, CEE, CGI-20, GET4, TRC35
Pfam: Golgi to ER traffic protein 4
InterPro:
Golgi to ER traffic protein 4 homolog in PDB entry 7ru9, assembly 1, front view.
Golgi to ER traffic protein 4 homolog in PDB entry 7ru9, assembly 1, side view.
Golgi to ER traffic protein 4 homolog in PDB entry 7ru9, assembly 1, top view.
Molecule details ›

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Large proline-rich protein BAG6
Chains: D, G
Length: 132 amino acids
Theoretical weight: 14.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46379 (Residues: 1004-1132; Coverage: 11%)
Gene names: BAG6, BAT3, G3
Pfam: Bag6, BAG-similar domain
Large proline-rich protein BAG6 in PDB entry 7ru9, assembly 1, front view.
Large proline-rich protein BAG6 in PDB entry 7ru9, assembly 1, side view.
Large proline-rich protein BAG6 in PDB entry 7ru9, assembly 1, top view.
Molecule details ›

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Ubiquitin-like protein 4A
Chains: E, H
Length: 165 amino acids
Theoretical weight: 18.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11441 (Residues: 1-157; Coverage: 100%)
Gene names: DXS254E, GDX, UBL4, UBL4A
Pfam:
InterPro:
Ubiquitin-like protein 4A in PDB entry 7ru9, assembly 1, front view.
Ubiquitin-like protein 4A in PDB entry 7ru9, assembly 1, side view.
Ubiquitin-like protein 4A in PDB entry 7ru9, assembly 1, top view.
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