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H. neapolitanus carboxysomal rubisco/CsoSCA-peptide (1-50)complex

Electron Microscopy
2.07Å resolution
PDB entry 7snv coloured by chain, front view.
PDB entry 7snv coloured by chain, side view.
PDB entry 7snv coloured by chain, top view.
Source organism: Halothiobacillus neapolitanus c2
Assembly composition:
hetero 24-mer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 24-mer
Accessible surface area: 124144.53 Å2
Buried surface area: 91233.62 Å2
Dissociation area: 6,042.18 Å2
Dissociation energy (ΔGdiss): 18.41 kcal/mol
Dissociation entropy (TΔSdiss): 56.75 kcal/mol
Symmetry number: 8
PDBe Complex ID: PDB-CPX-215126

Macromolecules

Ribulose bisphosphate carboxylase large chain
Chain: A
Length: 482 amino acids
Theoretical weight: 53.83 KDa
Source organism: Halothiobacillus neapolitanus c2
Expression system: Escherichia coli
UniProt:
  • Canonical: O85040 (Residues: 2-473; Coverage: 100%)
Gene names: Hneap_0922, cbbL
Pfam:
Ribulose bisphosphate carboxylase large chain in PDB entry 7snv, assembly 1, front view.
Ribulose bisphosphate carboxylase large chain in PDB entry 7snv, assembly 1, side view.
Ribulose bisphosphate carboxylase large chain in PDB entry 7snv, assembly 1, top view.
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Ribulose bisphosphate carboxylase small subunit
Chain: B
Length: 110 amino acids
Theoretical weight: 12.87 KDa
Source organism: Halothiobacillus neapolitanus c2
Expression system: Escherichia coli
UniProt:
  • Canonical: P45686 (Residues: 1-110; Coverage: 100%)
Gene names: Hneap_0921, cbbS, rbcS
Pfam: Ribulose bisphosphate carboxylase, small chain
Ribulose bisphosphate carboxylase small subunit in PDB entry 7snv, assembly 1, front view.
Ribulose bisphosphate carboxylase small subunit in PDB entry 7snv, assembly 1, side view.
Ribulose bisphosphate carboxylase small subunit in PDB entry 7snv, assembly 1, top view.
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Carboxysome shell carbonic anhydrase
Chain: C
Length: 50 amino acids
Theoretical weight: 5.69 KDa
Source organism: Halothiobacillus neapolitanus c2
Expression system: Not provided
UniProt:
  • Canonical: O85042 (Residues: 1-50; Coverage: 10%)
Gene names: Hneap_0919, ORF1, csoS3
Carboxysome shell carbonic anhydrase in PDB entry 7snv, assembly 1, front view.
Carboxysome shell carbonic anhydrase in PDB entry 7snv, assembly 1, side view.
Carboxysome shell carbonic anhydrase in PDB entry 7snv, assembly 1, top view.
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