Structure analysis

Pre translocation intermediate stalled with viomycin and bound with EF-G in a GDP and Pi state (Structure III-vio)

Electron Microscopy
3.2Å resolution
Assembly composition:
hetero 59-mer (preferred)
Entry contents: 53 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 59-mer
Accessible surface area: 823091.18 Å2
Buried surface area: 280060.25 Å2
Dissociation area: 1,671.9 Å2
Dissociation energy (ΔGdiss): 1.64 kcal/mol
Dissociation entropy (TΔSdiss): 19.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-244074

Macromolecules

Chain: b
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli K-12

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Chain: c
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli K-12

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Chain: d
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli K-12

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Chain: e
Length: 177 amino acids
Theoretical weight: 20.07 KDa
Source organism: Escherichia coli K-12

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Chain: f
Length: 176 amino acids
Theoretical weight: 18.8 KDa
Source organism: Escherichia coli K-12

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Chain: g
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli K-12

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Chain: a
Length: 223 amino acids
Theoretical weight: 23.74 KDa
Source organism: Escherichia coli K-12

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Chain: i
Length: 141 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli K-12

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Chain: j
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli K-12

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Chain: k
Length: 122 amino acids
Theoretical weight: 13.45 KDa
Source organism: Escherichia coli K-12

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Chain: l
Length: 143 amino acids
Theoretical weight: 14.88 KDa
Source organism: Escherichia coli K-12

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Chain: m
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli K-12

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Chain: n
Length: 120 amino acids
Theoretical weight: 13.72 KDa
Source organism: Escherichia coli K-12

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Chain: o
Length: 116 amino acids
Theoretical weight: 12.66 KDa
Source organism: Escherichia coli K-12

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Chain: p
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli K-12

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Chain: q
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli K-12

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Chain: r
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli K-12

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Chain: s
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli K-12

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Chain: t
Length: 93 amino acids
Theoretical weight: 10.55 KDa
Source organism: Escherichia coli K-12

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Chain: u
Length: 102 amino acids
Theoretical weight: 11.08 KDa
Source organism: Escherichia coli K-12

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Chain: v
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli K-12

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Chain: w
Length: 75 amino acids
Theoretical weight: 8.17 KDa
Source organism: Escherichia coli K-12

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Chain: x
Length: 77 amino acids
Theoretical weight: 8.9 KDa
Source organism: Escherichia coli K-12

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Chain: y
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli K-12

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Chain: z
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Escherichia coli K-12

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Chain: A
Length: 66 amino acids
Theoretical weight: 7.52 KDa
Source organism: Escherichia coli K-12

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Chain: B
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli K-12

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Chain: C
Length: 50 amino acids
Theoretical weight: 5.81 KDa
Source organism: Escherichia coli K-12

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Chain: D
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli K-12

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Chain: E
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia coli K-12

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Chain: F
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli K-12

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Chain: H
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli K-12

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Chain: L
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli K-12

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Chain: N
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Escherichia coli K-12

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Chain: O
Length: 98 amino acids
Theoretical weight: 11.2 KDa
Source organism: Escherichia coli K-12

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Chain: R
Length: 114 amino acids
Theoretical weight: 12.63 KDa
Source organism: Escherichia coli K-12

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Chain: S
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli K-12

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Chain: X
Length: 79 amino acids
Theoretical weight: 9.06 KDa
Source organism: Escherichia coli K-12

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Chain: G
Length: 218 amino acids
Theoretical weight: 24.25 KDa
Source organism: Escherichia coli K-12

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Chain: I
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli K-12

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Chain: J
Length: 157 amino acids
Theoretical weight: 16.53 KDa
Source organism: Escherichia coli K-12

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Chain: K
Length: 100 amino acids
Theoretical weight: 11.67 KDa
Source organism: Escherichia coli K-12

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Chain: M
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli K-12

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Chain: P
Length: 116 amino acids
Theoretical weight: 12.39 KDa
Source organism: Escherichia coli K-12

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Chain: Q
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli K-12

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Chain: T
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli K-12

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Chain: U
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli K-12

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Chain: V
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli K-12

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Chain: W
Length: 65 amino acids
Theoretical weight: 7.61 KDa
Source organism: Escherichia coli K-12

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Chain: Y
Length: 85 amino acids
Theoretical weight: 9.51 KDa
Source organism: Escherichia coli K-12

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Chain: Z
Length: 65 amino acids
Theoretical weight: 7.76 KDa
Source organism: Escherichia coli K-12

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Chain: 8
Length: 697 amino acids
Theoretical weight: 76.9 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Chain: h
Length: 6 amino acids
Theoretical weight: 704 Da
Source organism: Streptomyces californicus
Expression system: Not provided

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Name: 23S ribosomal RNA
Representative chains: 1
Source organism: Escherichia coli K-12 [83333]
Length: 2903 nucleotides
Theoretical weight: 941.31 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: 2
Source organism: Escherichia coli K-12 [83333]
Length: 120 nucleotides
Theoretical weight: 38.81 KDa
Rfam: 5S ribosomal RNA

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Name: 16S ribosomal RNA
Representative chains: 3
Source organism: Escherichia coli K-12 [83333]
Length: 1539 nucleotides
Theoretical weight: 498.73 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: Messenger RNA
Representative chains: 4
Source organism: Escherichia coli K-12 [83333]
Expression system: Not provided
Length: 19 nucleotides
Theoretical weight: 6.21 KDa

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Name: tRNA fMet
Representative chains: 6
Source organism: Escherichia coli K-12 [83333]
Expression system: Not provided
Length: 77 nucleotides
Theoretical weight: 24.8 KDa
Rfam: tRNA

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Name: tRNA Pro
Representative chains: 5
Source organism: Escherichia coli K-12 [83333]
Expression system: Not provided
Length: 76 nucleotides
Theoretical weight: 24.53 KDa
Rfam: tRNA

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