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E. coli DnaB bound to three DnaG C-terminal domains, ssDNA, ADP and AlF4

Electron Microscopy
4.2Å resolution
PDB entry 7t22 coloured by chain, front view.
PDB entry 7t22 coloured by chain, side view.
PDB entry 7t22 coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
hetero decamer (preferred)
Entry contents: 2 distinct polypeptide molecules
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DnaB-DnaG primase-helicase complex and DNA
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Multimeric state: hetero decamer
Accessible surface area: 127294.75 Å2
Buried surface area: 50261.79 Å2
Dissociation area: 1,054.1 Å2
Dissociation energy (ΔGdiss): 3.46 kcal/mol
Dissociation entropy (TΔSdiss): 13.1 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-279185

Macromolecules

Replicative DNA helicase DnaB
Chains: A, B, C, D, E, F
Length: 471 amino acids
Theoretical weight: 52.41 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACB0 (Residues: 1-471; Coverage: 100%)
Gene names: JW4012, b4052, dnaB, groP, grpA
Pfam:
InterPro:
Replicative DNA helicase DnaB in PDB entry 7t22, assembly 1, front view.
Replicative DNA helicase DnaB in PDB entry 7t22, assembly 1, side view.
Replicative DNA helicase DnaB in PDB entry 7t22, assembly 1, top view.
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DNA primase
Chains: G, H, I
Length: 148 amino acids
Theoretical weight: 16.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABS5 (Residues: 434-581; Coverage: 26%)
Gene names: JW3038, b3066, dnaG, dnaP, parB
Pfam: DNA primase DnaG DnaB-binding
InterPro:
DNA primase in PDB entry 7t22, assembly 1, front view.
DNA primase in PDB entry 7t22, assembly 1, side view.
DNA primase in PDB entry 7t22, assembly 1, top view.
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DNA chain M
Name: DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')
Representative chains: M
Length: 20 nucleotides
Theoretical weight: 6.04 KDa
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') in PDB entry 7t22, assembly 1, front view.
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') in PDB entry 7t22, assembly 1, side view.
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') in PDB entry 7t22, assembly 1, top view.
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