› 7t9n

Electron Microscopy
2.9Å resolution

M22 Agonist Autoantibody bound to Human Thyrotropin receptor in complex with miniGs399 (composite structure)

Released:
DOI: 10.2210/pdb7t9n/pdb
PDB entry 7t9n coloured by chain, front view.
PDB entry 7t9n coloured by chain, side view.
PDB entry 7t9n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Autoantibody mimicry of hormone action at the thyrotropin receptor.
Faust B, Billesbølle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A
Nature (2022)
PMID: 35940205
Related structures: EMD-25763

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-224554 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
M22 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 234 amino acids
Theoretical weight: 25.07 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
M22 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 252 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 142 amino acids
Theoretical weight: 15.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Thyrotropin receptor Chain: R
Molecule details ›
Chain: R
Length: 702 amino acids
Theoretical weight: 79.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A0A0MTJ0 (Residues: 22-764; Coverage: 93%)
Gene name: TSHR
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: X
Molecule details ›
Chain: X
Length: 261 amino acids
Theoretical weight: 30.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 204-394; Coverage: 46%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: Y
Molecule details ›
Chain: Y
Length: 370 amino acids
Theoretical weight: 40.79 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Z
Molecule details ›
Chain: Z
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

2 bound ligands:
Ligand Z41 1 x Z41
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-25763
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

3 review citations

G protein-coupled receptors in cochlea: Potential therapeutic targets for hearing loss.
Ma et al. (2022)
2 more

1 mention without citation

Autoantibody mimicry of hormone action at the thyrotropin receptor.
Faust et al. (2022)