› 7tac

Electron Microscopy
3.6Å resolution

Cryo-EM structure of the (TGA3)2-(NPR1)2-(TGA3)2 complex

Released:
DOI: 10.2210/pdb7tac/pdb
PDB entry 7tac coloured by chain, front view.
PDB entry 7tac coloured by chain, side view.
PDB entry 7tac coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis of NPR1 in activating plant immunity.
Kumar S, Zavaliev R, Wu Q, Zhou Y, Cheng J, Dillard L, Powers J, Withers J, Zhao J, Guan Z, Borgnia MJ, Bartesaghi A, Dong X, Zhou P
Nature 605 561-566 (2022)
PMID: 35545668
Related structures: EMD-25769

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161059 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulatory protein NPR1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 609 amino acids
Theoretical weight: 67.89 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P93002 (Residues: 1-593; Coverage: 100%)
Gene names: At1g64280, F15H21.6, NIM1, NPR1, SAI1
Sequence domains:
Transcription factor TGA3 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 323 amino acids
Theoretical weight: 36.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39234 (Residues: 87-384; Coverage: 78%)
Gene names: At1g22070, BZIP22, F2E2.14, TGA3
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand PLM 4 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-25769
Expression system: Escherichia coli

Quick links

Citations

7 review citations

TGA transcription factors-Structural characteristics as basis for functional variability.
Tomaž et al. (2022)
6 more

1 mention without citation

Structural basis of NPR1 in activating plant immunity.
Kumar et al. (2022)