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Cryo-EM structure of the (TGA3)2-(NPR1)2-(TGA3)2 complex

Electron Microscopy
3.6Å resolution
PDB entry 7tac coloured by chain, front view.
PDB entry 7tac coloured by chain, side view.
PDB entry 7tac coloured by chain, top view.
Source organism: Arabidopsis thaliana
Assembly composition:
hetero hexamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Transcription factor TGA3 and Regulatory protein NPR1
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Multimeric state: hetero hexamer
Accessible surface area: 74625.37 Å2
Buried surface area: 13157.95 Å2
Dissociation area: 1,096.95 Å2
Dissociation energy (ΔGdiss): -15.1 kcal/mol
Dissociation entropy (TΔSdiss): 28.18 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-161059

Macromolecules

Regulatory protein NPR1
Chains: A, B
Length: 609 amino acids
Theoretical weight: 67.89 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P93002 (Residues: 1-593; Coverage: 100%)
Gene names: At1g64280, F15H21.6, NIM1, NPR1, SAI1
Pfam:
InterPro:
Regulatory protein NPR1 in PDB entry 7tac, assembly 1, front view.
Regulatory protein NPR1 in PDB entry 7tac, assembly 1, side view.
Regulatory protein NPR1 in PDB entry 7tac, assembly 1, top view.
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Transcription factor TGA3
Chains: C, D, E, F
Length: 323 amino acids
Theoretical weight: 36.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39234 (Residues: 87-384; Coverage: 78%)
Gene names: At1g22070, BZIP22, F2E2.14, TGA3
Pfam:
InterPro:
Transcription factor TGA3 in PDB entry 7tac, assembly 1, front view.
Transcription factor TGA3 in PDB entry 7tac, assembly 1, side view.
Transcription factor TGA3 in PDB entry 7tac, assembly 1, top view.
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