7tco

Electron Microscopy
4.19Å resolution

Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.G458Y.SOSIP.664 with high-mannose glycans

Released:
Entry authors: Manne K, Henderson R, Acharya P
Related structures: EMD-25815

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-224856 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (8 distinct):
Envelope glycoprotein gp160 Chains: A, E, M
Molecule details ›
Chains: A, E, M
Length: 461 amino acids
Theoretical weight: 52.02 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: M4M0W3 (Residues: 31-488; Coverage: 54%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, F, N
Molecule details ›
Chains: B, F, N
Length: 160 amino acids
Theoretical weight: 18.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 508-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
CH235.12 Fab Heavy Chain Chains: C, G, O
Molecule details ›
Chains: C, G, O
Length: 225 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CH235.12 Fab Light Chain Chains: D, H, P
Molecule details ›
Chains: D, H, P
Length: 213 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.19Å
Relevant EMDB volumes: EMD-25815
Expression system: Homo sapiens