› 7tj5

Electron Microscopy
2.41Å resolution

SthK closed state, cAMP-bound in the presence of POPA

Released:
DOI: 10.2210/pdb7tj5/pdb
PDB entry 7tj5 coloured by chain, front view.
PDB entry 7tj5 coloured by chain, side view.
PDB entry 7tj5 coloured by chain, top view.
Source organism: Spirochaeta thermophila DSM 6578
Primary publication:
Anionic lipids unlock the gates of select ion channels in the pacemaker family.
Schmidpeter PAM, Wu D, Rheinberger J, Riegelhaupt PM, Tang H, Robinson CV, Nimigean CM
Nat Struct Mol Biol 29 1092-1100 (2022)
PMID: 36352139
Related structures: EMD-25916

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-binding domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 51.12 KDa
Source organism: Spirochaeta thermophila DSM 6578
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0GA88 (Residues: 1-420; Coverage: 98%)
Gene name: Spith_0644
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CMP 4 x CMP
Ligand D21 28 x D21
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.41Å
Relevant EMDB volumes: EMD-25916
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Mass spectrometry of intact membrane proteins: shifting towards a more native-like context.
Oluwole et al. (2023)
8 other articles

2 mentions without citation

Structural basis of properties, mechanisms, and channelopathy of cyclic nucleotide-gated channels.
Hu et al. (2023)
1 more